EMD-38846

Single-particle
3.5 Å
EMD-38846 Deposition: 25/01/2024
Map released: 14/08/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-38846

Block 1 of PhiKZ capsid

EMD-38846

Single-particle
3.5 Å
EMD-38846 Deposition: 25/01/2024
Map released: 14/08/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Phikzvirus phiKZ
Sample: Phikzvirus phiKZ

Deposition Authors: Yang Y, Shao Q , Guo M , Han L, Zhao X , Wang A , Li X, Wang B, Pan J, Chen Z , Fokine A, Sun L , Fang Q
Capsid structure of bacteriophage Phi KZ provides insights into assembly and stabilization of jumbo phages.
Yang Y, Shao Q , Guo M , Han L, Zhao X , Wang A , Li X, Wang B, Pan JA , Chen Z , Fokine A, Sun L , Fang Q
(2024) Nat Commun , 15 , 6551 - 6551
PUBMED: 39095371
DOI: doi:10.1038/s41467-024-50811-1
ISSN: 2041-1723
Abstract:
Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in immunocompromised individuals. It holds potential to be used as an antimicrobial agent and as a model for uncovering basic phage biology. Although previous low-resolution structural studies have indicated that jumbo phages may have more complicated capsid structures than smaller phages such as HK97, the detailed structures and the assembly mechanism of their capsids remain largely unknown. Here, we report a 3.5-Å-resolution cryo-EM structure of the ΦKZ capsid. The structure unveiled ten minor capsid proteins, with some decorating the outer surface of the capsid and the others forming a complex network attached to the capsid's inner surface. This network seems to play roles in driving capsid assembly and capsid stabilization. Similar mechanisms of capsid assembly and stabilization are probably employed by many other jumbo viruses.