EMD-40070
Cryo-EM map of synthetic cage_O3_10 reconstructed without symmetry (C1)
EMD-40070
Single-particle7.4 Å
![EMD-40070](https://www.ebi.ac.uk/emdb/images/entry/EMD-40070/400_40070.gif)
Map released: 28/06/2023
Last modified: 24/04/2024
Sample Organism:
synthetic construct
Sample: Expandable de novo designed complex cage_O3_10
Deposition Authors: Coudray N
,
Redler R
,
Hsia Y
,
Huddy TF
,
Baker D
,
Ekiert D
,
Bhabha G
Sample: Expandable de novo designed complex cage_O3_10
Deposition Authors: Coudray N
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Blueprinting extendable nanomaterials with standardized protein blocks.
Huddy TF
,
Hsia Y
,
Kibler RD
,
Xu J,
Bethel N
,
Nagarajan D,
Redler R
,
Leung PJY
,
Weidle C,
Courbet A
,
Yang EC
,
Bera AK
,
Coudray N
,
Calise SJ
,
Davila-Hernandez FA,
Han HL
,
Carr KD
,
Li Z
,
McHugh R,
Reggiano G,
Kang A,
Sankaran B
,
Dickinson MS,
Coventry B,
Brunette TJ
,
Liu Y,
Dauparas J,
Borst AJ,
Ekiert D
,
Kollman JM
,
Bhabha G
,
Baker D
(2024) Nature , 627 , 898 - 904
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(2024) Nature , 627 , 898 - 904
Abstract:
A wooden house frame consists of many different lumber pieces, but because of the regularity of these building blocks, the structure can be designed using straightforward geometrical principles. The design of multicomponent protein assemblies, in comparison, has been much more complex, largely owing to the irregular shapes of protein structures1. Here we describe extendable linear, curved and angled protein building blocks, as well as inter-block interactions, that conform to specified geometric standards; assemblies designed using these blocks inherit their extendability and regular interaction surfaces, enabling them to be expanded or contracted by varying the number of modules, and reinforced with secondary struts. Using X-ray crystallography and electron microscopy, we validate nanomaterial designs ranging from simple polygonal and circular oligomers that can be concentrically nested, up to large polyhedral nanocages and unbounded straight 'train track' assemblies with reconfigurable sizes and geometries that can be readily blueprinted. Because of the complexity of protein structures and sequence-structure relationships, it has not previously been possible to build up large protein assemblies by deliberate placement of protein backbones onto a blank three-dimensional canvas; the simplicity and geometric regularity of our design platform now enables construction of protein nanomaterials according to 'back of an envelope' architectural blueprints.
A wooden house frame consists of many different lumber pieces, but because of the regularity of these building blocks, the structure can be designed using straightforward geometrical principles. The design of multicomponent protein assemblies, in comparison, has been much more complex, largely owing to the irregular shapes of protein structures1. Here we describe extendable linear, curved and angled protein building blocks, as well as inter-block interactions, that conform to specified geometric standards; assemblies designed using these blocks inherit their extendability and regular interaction surfaces, enabling them to be expanded or contracted by varying the number of modules, and reinforced with secondary struts. Using X-ray crystallography and electron microscopy, we validate nanomaterial designs ranging from simple polygonal and circular oligomers that can be concentrically nested, up to large polyhedral nanocages and unbounded straight 'train track' assemblies with reconfigurable sizes and geometries that can be readily blueprinted. Because of the complexity of protein structures and sequence-structure relationships, it has not previously been possible to build up large protein assemblies by deliberate placement of protein backbones onto a blank three-dimensional canvas; the simplicity and geometric regularity of our design platform now enables construction of protein nanomaterials according to 'back of an envelope' architectural blueprints.