EMD-41933

Single-particle
1.81 Å
EMD-41933 Deposition: 12/09/2023
Map released: 28/02/2024
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-41933

CryoEM map of horse spleen apoferritin determined as a reference for benchmarking square and rectangular apertures for cryo-EM

EMD-41933

Single-particle
1.81 Å
EMD-41933 Deposition: 12/09/2023
Map released: 28/02/2024
Last modified: 24/04/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Equus caballus
Sample: Apoferritin from equine spleen

Deposition Authors: Brown HG , Hanssen E
Square condenser apertures for square cameras in low-dose transmission electron microscopy.
Brown HG , Smith D, Wardle BC, Hanssen E
(2024) Nat Methods , 21 , 566 - 568
PUBMED: 38459386
DOI: doi:10.1038/s41592-024-02206-9
ISSN: 1548-7105
Abstract:
In transmission electron microscopy (TEM), cameras are square or rectangular but beams are round so the circular lobes irradiate adjacent areas, precluding further neighboring acquisition for beam-sensitive samples. We present condenser aperture plates with square and rectangular shapes that improve the efficiency of area usage by 70% and enhance montage imaging for beam-sensitive specimens. We demonstrate the compatibility of these condenser aperture plates with high-resolution cryogenic TEM by reconstructing a 1.8-Å map of equine apo-ferritin.