EMD-42119
Toxoplasma gondii apical end with ICMAP2 conditionally knocked down
EMD-42119
Tomography
Map released: 07/08/2024
Last modified: 07/08/2024
Sample Organism:
Toxoplasma gondii
Sample: Toxoplasma Gondii tachyzoite apical complex with ICMAP2 conditionally knocked down
Deposition Authors: Martinez M
,
Dos Santos Pacheco N
,
Chang Y-W
,
Soldati-Favre D
Sample: Toxoplasma Gondii tachyzoite apical complex with ICMAP2 conditionally knocked down
Deposition Authors: Martinez M
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Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins.
Dos Santos Pacheco N
,
Tell I Puig A
,
Guerin A
,
Martinez M
,
Maco B
,
Tosetti N,
Delgado-Betancourt E
,
Lunghi M
,
Striepen B
,
Chang YW
,
Soldati-Favre D
(2024) Nat Commun , 15 , 379 - 379
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(2024) Nat Commun , 15 , 379 - 379
Abstract:
In Apicomplexa, rhoptry discharge is essential for invasion and involves an apical vesicle (AV) docking one or two rhoptries to a macromolecular secretory apparatus. Toxoplasma gondii is armed with 10-12 rhoptries and 5-6 microtubule-associated vesicles (MVs) presumably for iterative rhoptry discharge. Here, we have addressed the localization and functional significance of two intraconoidal microtubule (ICMT)-associated proteins instrumental for invasion. Mechanistically, depletion of ICMAP2 leads to a dissociation of the ICMTs, their detachment from the conoid and dispersion of MVs and rhoptries. ICMAP3 exists in two isoforms that contribute to the control of the ICMTs length and the docking of the two rhoptries at the AV, respectively. This study illuminates the central role ICMTs play in scaffolding the discharge of multiple rhoptries. This process is instrumental for virulence in the mouse model of infection and in addition promotes sterile protection against T. gondii via the release of key effectors inducing immunity.
In Apicomplexa, rhoptry discharge is essential for invasion and involves an apical vesicle (AV) docking one or two rhoptries to a macromolecular secretory apparatus. Toxoplasma gondii is armed with 10-12 rhoptries and 5-6 microtubule-associated vesicles (MVs) presumably for iterative rhoptry discharge. Here, we have addressed the localization and functional significance of two intraconoidal microtubule (ICMT)-associated proteins instrumental for invasion. Mechanistically, depletion of ICMAP2 leads to a dissociation of the ICMTs, their detachment from the conoid and dispersion of MVs and rhoptries. ICMAP3 exists in two isoforms that contribute to the control of the ICMTs length and the docking of the two rhoptries at the AV, respectively. This study illuminates the central role ICMTs play in scaffolding the discharge of multiple rhoptries. This process is instrumental for virulence in the mouse model of infection and in addition promotes sterile protection against T. gondii via the release of key effectors inducing immunity.