EMD-50231

Tomography
EMD-50231 Deposition: 02/05/2024
Map released: 26/06/2024
Last modified: 24/07/2024
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EMD-50231

Cryo-tomogram of FIB-milled meiotic yeast cell containing mitochondria with filaments

EMD-50231

Tomography
EMD-50231 Deposition: 02/05/2024
Map released: 26/06/2024
Last modified: 24/07/2024
Overview Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae
Sample: Cryo FIB-milled meiotic yeast cells

Deposition Authors: Wettstein R , Hugener J , Gillet L, Hernandez-Armenta Y, Henggeler A , Xu J , Van Gerwen J, Wollweber F, Arter M, Aebersold R, Beltrao P, Pilhofer M, Matos J
Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast.
PUBMED: 38906138
DOI: doi:10.1016/j.devcel.2024.05.025
ISSN: 1534-5807
Abstract:
Sexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FoF1-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4ALDH2, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4ALDH2 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.