EMD-50415

Subtomogram averaging
10.2 Å
EMD-50415 Deposition: 24/05/2024
Map released: 17/07/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-50415

Subtomogram average of 80S ribosomes in S. cerevisiae under acute glucose starvation

EMD-50415

Subtomogram averaging
10.2 Å
EMD-50415 Deposition: 24/05/2024
Map released: 17/07/2024
Last modified: 14/08/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: S.cerevisiae
Sample: Cryo-focused ion beam milled lamellae of S.cerevisiae cells under acute glucose starvation
Raw data: EMPIAR-12104

Deposition Authors: Spindler MC , Mahamid J
Polysome collapse and RNA condensation fluidize the cytoplasm.
Xie Y, Shu T , Liu T, Spindler MC , Mahamid J , Hocky GM , Gresham D, Holt LJ
(2024) Mol Cell , 84 , 2698 - 2716.e9
PUBMED: 39059370
DOI: doi:10.1016/j.molcel.2024.06.024
ISSN: 1097-2765
ASTM: MOCEFL
Abstract:
The cell interior is packed with macromolecules of mesoscale size, and this crowded milieu significantly influences cellular physiology. Cellular stress responses almost universally lead to inhibition of translation, resulting in polysome collapse and release of mRNA. The released mRNA molecules condense with RNA-binding proteins to form ribonucleoprotein (RNP) condensates known as processing bodies and stress granules. Here, we show that polysome collapse and condensation of RNA transiently fluidize the cytoplasm, and coarse-grained molecular dynamic simulations support this as a minimal mechanism for the observed biophysical changes. Increased mesoscale diffusivity correlates with the efficient formation of quality control bodies (Q-bodies), membraneless organelles that compartmentalize misfolded peptides during stress. Synthetic, light-induced RNA condensation also fluidizes the cytoplasm. Together, our study reveals a functional role for stress-induced translation inhibition and formation of RNP condensates in modulating the physical properties of the cytoplasm to enable efficient response of cells to stress conditions.