EMD-50556

Subtomogram averaging
30.0 Å
EMD-50556 Deposition: 06/06/2024
Map released: 30/10/2024
Last modified: 30/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-50556

subtomogram averaging of the baseplate of a type 6 secretion system in Aureispira sp. CCB-QB1

EMD-50556

Subtomogram averaging
30.0 Å
EMD-50556 Deposition: 06/06/2024
Map released: 30/10/2024
Last modified: 30/10/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Aureispira sp. CCB-QB1
Sample: Extracellular antenna of type 6 secretion system in Aureispira sp. CCB-QB1

Deposition Authors: Lien Y-W, Amendola D , Lee KS , Bartlau N , Xu J , Furusawa G, Polz MF , Stocker R , Weiss GL , Pilhofer M
Mechanism of bacterial predation via ixotrophy.
Lien YW , Amendola D , Lee KS , Bartlau N , Xu J , Furusawa G, Polz MF , Stocker R , Weiss GL , Pilhofer M
(2024) Science , 386 , eadp0614 - eadp0614
PUBMED: 39418385
DOI: doi:10.1126/science.adp0614
ISSN: 1095-9203
ASTM: SCIEAS
Abstract:
Ixotrophy is a contact-dependent predatory strategy of filamentous bacteria in aquatic environments for which the molecular mechanism remains unknown. We show that predator-prey contact can be established by gliding motility or extracellular assemblages we call "grappling hooks." Cryo-electron microscopy identified the grappling hooks as heptamers of a type IX secretion system substrate. After close predator-prey contact is established, cryo-electron tomography and functional assays showed that puncturing by a type VI secretion system mediated killing. Single-cell analyses with stable isotope-labeled prey revealed that prey components are taken up by the attacker. Depending on nutrient availability, insertion sequence elements toggle the activity of ixotrophy. A marine metagenomic time series shows coupled dynamics of ixotrophic bacteria and prey. We found that the mechanism of ixotrophy involves multiple cellular machineries, is conserved, and may shape microbial populations in the environment.