EMD-5134

Single-particle
8.0 Å
EMD-5134 Deposition: 19/10/2009
Map released: 02/11/2009
Last modified: 10/01/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5134

TBP-containing S. pombe TFIID

EMD-5134

Single-particle
8.0 Å
EMD-5134 Deposition: 19/10/2009
Map released: 02/11/2009
Last modified: 10/01/2011
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample: Native S. pombe TFIID

Deposition Authors: Elmlund H , Baraznenok V, Linder T , Szilagyi Z, Rofougaran R, Hofer A , Hebert H, Lindahl M, Gustafsson CM
Cryo-EM reveals promoter DNA binding and conformational flexibility of the general transcription factor TFIID.
Abstract:
The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the TFIID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.