EMD-5942

Single-particle
6.1 Å
EMD-5942 Deposition: 09/04/2014
Map released: 11/06/2014
Last modified: 07/10/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-5942

Cryo-EM Map of a yeast ribosome bound to the TSV IRES (Class II)

EMD-5942

Single-particle
6.1 Å
EMD-5942 Deposition: 09/04/2014
Map released: 11/06/2014
Last modified: 07/10/2015
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Saccharomyces cerevisiae, Taura syndrome virus
Sample: Saccharomyces cerevisiae 80S ribosome bound with TSV IRES
Fitted models: 3j6x (Avg. Q-score: 0.203)
Raw data: EMPIAR-10015

Deposition Authors: Koh CS, Brilot AF , Grigorieff N , Korostelev AA
Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center.
Koh CS, Brilot AF , Grigorieff N , Korostelev AA
(2014) PNAS , 111 , 9139 - 9144
Abstract:
In cap-dependent translation initiation, the open reading frame (ORF) of mRNA is established by the placement of the AUG start codon and initiator tRNA in the ribosomal peptidyl (P) site. Internal ribosome entry sites (IRESs) promote translation of mRNAs in a cap-independent manner. We report two structures of the ribosome-bound Taura syndrome virus (TSV) IRES belonging to the family of Dicistroviridae intergenic IRESs. Intersubunit rotational states differ in these structures, suggesting that ribosome dynamics play a role in IRES translocation. Pseudoknot I of the IRES occupies the ribosomal decoding center at the aminoacyl (A) site in a manner resembling that of the tRNA anticodon-mRNA codon. The structures reveal that the TSV IRES initiates translation by a previously unseen mechanism, which is conceptually distinct from initiator tRNA-dependent mechanisms. Specifically, the ORF of the IRES-driven mRNA is established by the placement of the preceding tRNA-mRNA-like structure in the A site, whereas the 40S P site remains unoccupied during this initial step.