EMD-60712

Helical reconstruction
3.6 Å
EMD-60712 Deposition: 08/07/2024
Map released: 04/09/2024
Last modified: 04/09/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-60712

Structure of bacteriophage T5 tail tube

EMD-60712

Helical reconstruction
3.6 Å
EMD-60712 Deposition: 08/07/2024
Map released: 04/09/2024
Last modified: 04/09/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Escherichia phage T5
Sample: Escherichia phage T5
Fitted models: 9iny

Deposition Authors: Peng YN, Liu HR
Structures of Mature and Urea-Treated Empty Bacteriophage T5: Insights into Siphophage Infection and DNA Ejection.
Peng Y , Tang H, Xiao H, Chen W, Song J, Zheng J, Liu H
(2024) Int J Mol Sci , 25
PUBMED: 39126049
DOI: doi:10.3390/ijms25158479
ISSN: 1422-0067
Abstract:
T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of T5 before and after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine the structures of mature T5 (a laboratory-adapted, fiberless T5 mutant) and urea-treated empty T5 (lacking the tip complex) at near-atomic resolutions. Atomic models of the head, connector complex, tail tube, and tail tip were built for mature T5, and atomic models of the connector complex, comprising the portal protein pb7, adaptor protein p144, and tail terminator protein p142, were built for urea-treated empty T5. Our findings revealed that the aforementioned proteins did not undergo global conformational changes before and after DNA ejection, indicating that these structural features were conserved among most myophages and siphophages. The present study elucidates the underlying mechanisms of siphophage infection and DNA ejection.