EMD-9171

Subtomogram averaging
23.1 Å
EMD-9171 Deposition: 01/10/2018
Map released: 27/02/2019
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9171

Electron cryo-tomography and subtomogram averaging of microtubule triplet from procentriole

EMD-9171

Subtomogram averaging
23.1 Å
EMD-9171 Deposition: 01/10/2018
Map released: 27/02/2019
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Chlamydomonas reinhardtii
Sample: Centriole

Deposition Authors: Li S, Fernandez JJ, Marshall W, Agard DA
Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism.
Li S , Fernandez JJ , Marshall WF , Agard DA
(2019) eLife , 8
PUBMED: 30741631
DOI: doi:10.7554/eLife.43434
ISSN: 2050-084X
Abstract:
Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii. We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly.