EMD-9179

Single-particle
29.5 Å
EMD-9179 Deposition: 03/10/2018
Map released: 05/06/2019
Last modified: 05/06/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links

EMD-9179

Negative Stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from non-human primate RQq16

EMD-9179

Single-particle
29.5 Å
EMD-9179 Deposition: 03/10/2018
Map released: 05/06/2019
Last modified: 05/06/2019
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Macaca
Sample: Negative Stain EM map of polyclonal serum in complex with BG505 SOSIP.664 from non-human primate RQq16

Deposition Authors: Nogal B, Ward AB
Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance.
PUBMED: 31080066
DOI: doi:10.1016/j.cell.2019.04.012
ISSN: 1097-4172
Abstract:
Conventional immunization strategies will likely be insufficient for the development of a broadly neutralizing antibody (bnAb) vaccine for HIV or other difficult pathogens because of the immunological hurdles posed, including B cell immunodominance and germinal center (GC) quantity and quality. We found that two independent methods of slow delivery immunization of rhesus monkeys (RMs) resulted in more robust T follicular helper (TFH) cell responses and GC B cells with improved Env-binding, tracked by longitudinal fine needle aspirates. Improved GCs correlated with the development of >20-fold higher titers of autologous nAbs. Using a new RM genomic immunoglobulin locus reference, we identified differential IgV gene use between immunization modalities. Ab mapping demonstrated targeting of immunodominant non-neutralizing epitopes by conventional bolus-immunized animals, whereas slow delivery-immunized animals targeted a more diverse set of epitopes. Thus, alternative immunization strategies can enhance nAb development by altering GCs and modulating the immunodominance of non-neutralizing epitopes.