EMD-9793
The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus
EMD-9793
Single-particle4.8 Å
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Map released: 07/08/2019
Last modified: 27/03/2024
Sample Organism:
Avian avulavirus 1,
Escherichia coli
Sample: Ternary complex of nucleoprotein-RNA of Newcastle disease virus
Fitted models: 6jc3 (Avg. Q-score: 0.281)
Deposition Authors: Song X
,
Shan H
Sample: Ternary complex of nucleoprotein-RNA of Newcastle disease virus
Fitted models: 6jc3 (Avg. Q-score: 0.281)
Deposition Authors: Song X
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Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.
Song X
,
Shan H,
Zhu Y,
Hu S,
Xue L,
Chen Y
,
Ding W
,
Niu T,
Gu J,
Ouyang S,
Shen QT,
Liu ZJ
(2019) eLife , 8
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(2019) eLife , 8
Abstract:
Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.
Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.