EMD-9793

Single-particle
4.8 Å
EMD-9793 Deposition: 28/01/2019
Map released: 07/08/2019
Last modified: 27/03/2024
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EMD-9793

The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus

EMD-9793

Single-particle
4.8 Å
EMD-9793 Deposition: 28/01/2019
Map released: 07/08/2019
Last modified: 27/03/2024
Overview 3D View Sample Experiment Validation Volume Browser Additional data Links
Sample Organism: Avian avulavirus 1, Escherichia coli
Sample: Ternary complex of nucleoprotein-RNA of Newcastle disease virus
Fitted models: 6jc3 (Avg. Q-score: 0.281)

Deposition Authors: Song X , Shan H
Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.
PUBMED: 31290740
DOI: doi:10.7554/eLife.45057
ISSN: 2050-084X
Abstract:
Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.