Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8pv8
Q-score: 0.559
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi3 (47 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l29 (7 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l41-a (3 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (9 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Protein sda1 (86 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 2 - pre-5s rotation with rix1 complex - composite structure (Complex)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein rix1 (84 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 5 - pre-5S rotation - L1 inward - composite structure
Resolution: 2.55 Å
EM Method: Single-particle
Fitted PDBs: 8pvk
Q-score: 0.606
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 5 - pre-5s rotation - l1 inward - composite structure (Complex)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Utp30 (43 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum pre-60S State 7 - pre-5S rotation lacking Utp30/ITS2 - composite structure
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 8pvl
Q-score: 0.662
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nop53 (50 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Putative rna-binding protein (45 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Chaetomium thermophilum pre-60s state 7 - pre-5s rotation lacking utp30/its2 - composite structure (Complex)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc ion (65 Da, Ligand)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (25 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome production factor 2 homolog (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Its2 (52 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pescadillo homolog (77 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis regulatory protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Chaetomium thermophilum Rix1-complex
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8ptw
Q-score: 0.549
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Chaetomium thermophilum low-resolution Rixosome
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]