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* AND author_name:"Berninghausen O" AND author_orcid:"0000-0003-4442-377x" AND xref_links:"scop2"
Structure of a human ABCE1-bound 43S pre-initiation complex - State II
Release date: Oct. 7, 2020
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6zvj
Q-score: 0.365
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6zvj
Q-score: 0.365
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
Sample:
- 18s ribosomal rna (555 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s3 (24 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 40s ribosomal protein sa (24 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit l (44 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s24 (14 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit b (65 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s30 (5 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit k (24 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit f (29 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s26 (11 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit i (35 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- Receptor of activated protein c kinase 1 (34 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s19 (15 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (7 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit m (41 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (24 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s25 (7 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s20 (11 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s14 (13 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 1 (10 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s10 (11 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (23 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s21 (8 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit c (86 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s6 (25 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (21 kDa, Protein from Homo sapiens)
- Rna recognition motif (unknown) (5 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s7 (20 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4, x isoform (28 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit e (50 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s9 (21 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Eukaryotic translation initiation factor 3 subunit d (61 kDa, Protein from Homo sapiens)
- Abce1-bound 43s initiation complex (Complex from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit a (85 kDa, Protein from Homo sapiens)
- Atp-binding cassette sub-family e member 1 (66 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s2 (23 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s12 (12 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s11 (16 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit h (36 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s18 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15 (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s16 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (6 kDa, Protein from Homo sapiens)
Cryo-EM structure of the 90S pre-ribosome from Chaetomium thermophilum
Release date: Oct. 11, 2017
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 5oql
Q-score: 0.382
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 5oql
Q-score: 0.382
Cheng J, Kellner N, Berninghausen O, Hurt E, Beckmann R
Nat Struct Mol Biol (2017) 24 pp. 954-964 [ DOI: doi:10.1038/nsmb.3476 Pubmed: 28967883 ]
Sample:
- Sof1 (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp10 (198 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp12 (107 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bms1 (134 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Snu13 (13 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp4 (97 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp3 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 35s rrna (827 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp30 (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative nucleolar protein (57 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp7 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop1 (32 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp2 (103 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp13 (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp18 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 snorna (87 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Faf1 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Imp3 (21 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- The 90s pre-ribosomne (Complex from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Emg1 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Zinc ion (65 Da, Ligand)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp17 (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp15 (60 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s7-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp24 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp6 (46 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp14 (104 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop58 (64 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna cytidine acetyltransferase (119 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))