Shi J, Feng Z, Song Q, Wen A, Liu T, Xu L, Ye Z, Xu S, Gao F, Xiao L, Zhu J, Das K, Zhao G, Li J, Feng Y, Lin W
Nat Commun (2025) 16 pp. 1573- [ DOI: doi:10.1038/s41467-025-56697-x ]
- Mycobacterium tuberculosis transcription activation complex with unphosphated phop (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Template strand dna (33 kDa, DNA from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Non-template strand dna (33 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis)
Shi J, Feng Z, Song Q, Wen A, Liu T, Xu L, Ye Z, Xu S, Gao F, Xiao L, Zhu J, Das K, Zhao G, Li J, Feng Y, Lin W
Nat Commun (2025) 16 pp. 1573- [ DOI: doi:10.1038/s41467-025-56697-x ]
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Possible two component system response transcriptional positive regulator phop (30 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Mycobacterium tuberculosis transcription activation complex with two phop molecules (Complex from Mycobacterium tuberculosis)
- Template strand dna (23 kDa, DNA from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Non-template strand dna (23 kDa, DNA from Mycobacterium tuberculosis H37Rv)
Shi J, Feng Z, Song Q, Wen A, Liu T, Xu L, Ye Z, Xu S, Gao F, Xiao L, Zhu J, Das K, Zhao G, Li J, Feng Y, Lin W
Nat Commun (2025) 16 pp. 1573- [ DOI: doi:10.1038/s41467-025-56697-x ]
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Mycobacterium tuberculosis transcription activation complex with four phop molecules (composite map) (Complex from Mycobacterium tuberculosis)
- Template strand dna of the promoter (30 kDa, DNA from Mycobacterium tuberculosis H37Rv)
- Possible two component system response transcriptional positive regulator phop (27 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Non-template strand dna of the promoter (30 kDa, DNA from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Shi J, Feng Z, Song Q, Wen A, Liu T, Xu L, Ye Z, Xu S, Gao F, Xiao L, Zhu J, Das K, Zhao G, Li J, Feng Y, Lin W
Nat Commun (2025) 16 pp. 1573- [ DOI: doi:10.1038/s41467-025-56697-x ]
- Dna-directed rna polymerase subunit beta' (146 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Template strand dna of the promoter (33 kDa, DNA from Mycobacterium tuberculosis H37Rv)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (57 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Mycobacterium tuberculosis transcription activation complex with six phop molecules (composite map) (Complex from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Possible two component system response transcriptional positive regulator phop (27 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Non-template strand dna of the promoter (33 kDa, DNA from Mycobacterium tuberculosis H37Rv)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis H37Rv)
Cryo-EM Structure of the 3HO-HCAR3-Gi complex
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8jef
Q-score: 0.517
Ye F, Pan X, Zhang Z, Xiang X, Li X, Zhang B, Ning P, Liu A, Wang Q, Gong K, Li J, Zhu L, Qian C, Chen G, Du Y
Cell Rep (2024) 43 pp. 114895-114895 [ DOI: doi:10.1016/j.celrep.2024.114895 Pubmed: 39427321 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (3s)-3-hydroxyoctanoic acid (160 Da, Ligand)
- Cryo-em structure of the apo hcar3-gi complex (Complex from Homo sapiens)
- Hydroxycarboxylic acid receptor 3 (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
Cryo-EM Structure of the compuond 5c-HCAR3-Gi complex
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 8jei
Q-score: 0.535
Ye F, Pan X, Zhang Z, Xiang X, Li X, Zhang B, Ning P, Liu A, Wang Q, Gong K, Li J, Zhu L, Qian C, Chen G, Du Y
Cell Rep (2024) 43 pp. 114895-114895 [ DOI: doi:10.1016/j.celrep.2024.114895 Pubmed: 39427321 ]
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-nitro-4-(propylamino)benzoic acid (224 Da, Ligand)
- Hydroxycarboxylic acid receptor 3 (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cryo-em structure of the compound 5c-hcar3-gi complex (Complex from Homo sapiens)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8k0c
Q-score: 0.397
Fan P, Sun M, Zhang X, Zhang H, Liu Y, Yao Y, Li M, Fang T, Sun B, Chen Z, Chi X, Chen L, Peng C, Chen Z, Zhang G, Ren Y, Liu Z, Li Y, Li J, Li E, Guan W, Li S, Gong R, Zhang K, Yu C, Chiu S
Nat Commun (2024) 15 pp. 4330-4330 [ DOI: doi:10.1038/s41467-024-48601-w Pubmed: 38773072 ]
- Heavy chain of 1e5 fab fragments (26 kDa, Protein from Macaca mulatta)
- Glycoprotein g (56 kDa, Protein from Nipah virus)
- Cryo-em structure of conformation 1 of complex of nipah virus attachment glycoprotein g with 1e5 neutralizing antibody (Complex from Nipah virus)
- Light chain of 1e5 fab fragments (23 kDa, Protein from Macaca mulatta)
- Glycoprotein g (6 kDa, Protein from Nipah virus)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8k0d
Q-score: 0.461
Fan P, Sun M, Zhang X, Zhang H, Liu Y, Yao Y, Li M, Fang T, Sun B, Chen Z, Chi X, Chen L, Peng C, Chen Z, Zhang G, Ren Y, Liu Z, Li Y, Li J, Li E, Guan W, Li S, Gong R, Zhang K, Yu C, Chiu S
Nat Commun (2024) 15 pp. 4330-4330 [ DOI: doi:10.1038/s41467-024-48601-w Pubmed: 38773072 ]
- Cryo-em structure of conformation 2 of complex of nipah virus attachment g with 1e5 neutralizing antibody (Complex from Nipah virus)
- The light chain of 1e5 (23 kDa, Protein from Macaca mulatta)
- Glycoprotein g (45 kDa, Protein from Nipah virus)
- The heavy chain of 1e5 (26 kDa, Protein from Macaca mulatta)
Cryo-EM structure of human HCAR2-Gi complex without ligand (apo state)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8ij3
Q-score: 0.506
Pan X, Ye F, Ning P, Zhang Z, Li X, Zhang B, Wang Q, Chen G, Gao W, Qiu C, Wu Z, Li J, Zhu L, Xia J, Gong K, Du Y
Cell Discov (2023) 9 pp. 118-118 [ DOI: doi:10.1038/s41421-023-00610-7 Pubmed: 38012147 ]
- Hydroxycarboxylic acid receptor 2 (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cryo-em structure of human hcar2-gi complex without ligand (apo state) (Complex from Homo sapiens)
Cryo-EM structure of human HCAR2-Gi complex with niacin
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8ija
Q-score: 0.574
Pan X, Ye F, Ning P, Zhang Z, Li X, Zhang B, Wang Q, Chen G, Gao W, Qiu C, Wu Z, Li J, Zhu L, Xia J, Gong K, Du Y
Cell Discov (2023) 9 pp. 118-118 [ DOI: doi:10.1038/s41421-023-00610-7 Pubmed: 38012147 ]
- Cryo-em structure of human hcar2-gi complex with niacin (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Hydroxycarboxylic acid receptor 2 (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Nicotinic acid (123 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)