Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8xyd
Q-score: 0.366
Fan W, Xu Y, He X, Luo P, Zhu J, Li J, Wang R, Yuan Q, Wu K, Hu W, Zhao Y, Xu S, Cheng X, Wang Y, Xu HE, Zhuang Y
Cell Rep (2024) 43 pp. 114422-114422 [ DOI: doi:10.1016/j.celrep.2024.114422 Pubmed: 38943642 ]
- Ptafr-paf g protein complex (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Platelet-activating factor receptor (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (2r)-2-(acetyloxy)-3-(hexadecyloxy)propyl 2-(trimethylammonio)ethyl phosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of prolactin-releasing peptide recognition with Gi
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8zps
Q-score: 0.406
Li Y, Yuan Q, He X, Zhang Y, You C, Wu C, Li J, Xu HE, Zhao LH
Cell Discov (2024) 10 pp. 91-91 [ DOI: doi:10.1038/s41421-024-00724-6 Pubmed: 39223120 ]
- Prolactin-releasing peptide receptor (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Molecular mechanism of prolactin-releasing peptide recognition with gi (160 kDa, Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Prolactin-releasing peptide prrp20 (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of prolactin-releasing peptide recognition with Gq
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8zpt
Q-score: 0.432
Li Y, Yuan Q, He X, Zhang Y, You C, Wu C, Li J, Xu HE, Zhao LH
Cell Discov (2024) 10 pp. 91-91 [ DOI: doi:10.1038/s41421-024-00724-6 Pubmed: 39223120 ]
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Prolactin-releasing peptide receptor (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(324) subunit alpha-1, (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Molecular mechanism of prolactin-releasing peptide recognition with gq (160 kDa, Complex from Homo sapiens)
- Prolactin-releasing peptide prrp20 (2 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the cortistatin 17-bound Somatostatin receptor 5-Gi protein complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8x8l
Q-score: 0.493
Li J, You C, Li Y, Li C, Fan W, Chen Z, Hu W, Wu K, Xu HE, Zhao LH
PNAS (2024) 121 pp. e2321710121-e2321710121 [ Pubmed: 38885377 DOI: doi:10.1073/pnas.2321710121 ]
- Cortistatin (2 kDa, Protein from Homo sapiens)
- Cortistatin 17-bound somatostatin receptor 5-gi protein complex (Complex from Homo sapiens)
- Somatostatin receptor type 5 (39 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the octreotide-bound Somatostatin receptor 5-Gi protein complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8x8n
Q-score: 0.483
Li J, You C, Li Y, Li C, Fan W, Chen Z, Hu W, Wu K, Xu HE, Zhao LH
PNAS (2024) 121 pp. e2321710121-e2321710121 [ Pubmed: 38885377 DOI: doi:10.1073/pnas.2321710121 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Octreotide (1 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 5 (39 kDa, Protein from Homo sapiens)
- Octreotide-bound somatostatin receptor 5-gi protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
human KCNQ2-CaM-Ebio1 complex in the presence of PIP2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ijk
Q-score: 0.383
Zhang S, Ma D, Wang K, Li Y, Yang Z, Li X, Li J, He J, Mei L, Ye Y, Chen Z, Shen J, Hou P, Guo J, Zhang Q, Yang H
Nat Chem Biol (2024) 20 pp. 847-856 [ Pubmed: 38167918 DOI: doi:10.1038/s41589-023-01515-y ]
- Human kcnq2-cam-ebio1 complex in the presence of pip2 (Complex from Homo sapiens)
- Potassium voltage-gated channel subfamily kqt member 2 (73 kDa, Protein from Homo sapiens)
- N-(1,2-dihydroacenaphthylen-5-yl)-4-fluoranyl-benzamide (291 Da, Ligand)
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
human KCNQ2-CaM-Ebio1-S1 complex in the presence of PIP2
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8x43
Q-score: 0.328
Zhang S, Ma D, Wang K, Li Y, Yang Z, Li X, Li J, He J, Mei L, Ye Y, Chen Z, Shen J, Hou P, Guo J, Zhang Q, Yang H
Nat Chem Biol (2024) 20 pp. 847-856 [ Pubmed: 38167918 DOI: doi:10.1038/s41589-023-01515-y ]
- Human kcnq2-cam-ebio1-s1 complex in the presence of pip2 (Cellular component from Homo sapiens)
- Potassium voltage-gated channel subfamily kqt member 2 (73 kDa, Protein from Homo sapiens)
- N-(4-azanyl-1,2-dihydroacenaphthylen-5-yl)-4-fluoranyl-benzamide (306 Da, Ligand)
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Staurosporine (466 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Staurosporine (466 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)