Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: 10.26508/lsa.202201527 ]
Image sets:
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra
- Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4
- Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4
Structure of porcine V-ATPase with mEAK7 and SidK, Rotary state 2
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7u8o
Q-score: 0.371
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- Tldc protein meak-7 (50 kDa, Protein from Sus scrofa)
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
- V-atpase in complex with recombinant sidk and recombinant meak-7 (Complex from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7u8p
Q-score: 0.334
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Porcine kidney v-atpase with sidk, rotary state 1 (Complex from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7u8q
Q-score: 0.247
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Porcine kidney v-atpase with sidk, rotary state 2 (Complex from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 3
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7u8r
Q-score: 0.301
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Porcine kidney v-atpase with sidk, rotary state 3 (Complex from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Citrus V-ATPase State 1, Highest-Resolution Class
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022) 30 pp. 1403-1410.e4 [ DOI: doi:10.1016/j.str.2022.07.006 Pubmed: 36041457 ]
- Citrus v-atpase (Complex from Citrus limon)
Citrus V-ATPase State 1, H in contact with subunit a
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7uw9
Q-score: 0.311
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022) 30 pp. 1403- [ DOI: doi:10.1016/j.str.2022.07.006 Pubmed: 36041457 ]
- V-type proton atpase subunit c (16 kDa, Protein from Citrus limon)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Citrus limon)
- V-type proton atpase subunit g (12 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap2 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d (29 kDa, Protein from Citrus limon)
- V-type proton atpase subunit b2 (54 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c" (18 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e (26 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e1 (7 kDa, Protein from Citrus limon)
- Citrus v-atpase (Complex from Citrus limon)
- V-type proton atpase subunit h (51 kDa, Protein from Citrus limon)
- V-type proton atpase subunit f (14 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d2 (40 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap1 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit a3 (92 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c (42 kDa, Protein from Citrus limon)
Citrus V-ATPase State 1, H in contact with subunits AB
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7uwa
Q-score: 0.311
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022) 30 pp. 1403- [ DOI: doi:10.1016/j.str.2022.07.006 Pubmed: 36041457 ]
- V-type proton atpase subunit c (16 kDa, Protein from Citrus limon)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Citrus limon)
- V-type proton atpase subunit g (12 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap2 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d (29 kDa, Protein from Citrus limon)
- V-type proton atpase subunit b2 (54 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c" (18 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e (26 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e1 (7 kDa, Protein from Citrus limon)
- Citrus v-atpase (Complex from Citrus limon)
- V-type proton atpase subunit f (14 kDa, Protein from Citrus limon)
- V-type proton atpase subunit h (51 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d2 (40 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap1 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit a3 (92 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c (42 kDa, Protein from Citrus limon)
Citrus V-ATPase State 2, Highest-Resolution Class
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7uwb
Q-score: 0.378
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022) 30 pp. 1403- [ DOI: doi:10.1016/j.str.2022.07.006 Pubmed: 36041457 ]
- V-type proton atpase subunit c (16 kDa, Protein from Citrus limon)
- V-type proton atpase subunit g (12 kDa, Protein from Citrus limon)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap2 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d (29 kDa, Protein from Citrus limon)
- V-type proton atpase subunit b2 (54 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c" (18 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e (26 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e1 (7 kDa, Protein from Citrus limon)
- Citrus v-atpase (Complex from Citrus limon)
- V-type proton atpase subunit f (14 kDa, Protein from Citrus limon)
- V-type proton atpase subunit h (51 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d2 (40 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap1 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit a3 (92 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c (42 kDa, Protein from Citrus limon)
Citrus V-ATPase State 2, H in contact with subunit a
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7uwc
Q-score: 0.343
Tan YZ, Keon KA, Abdelaziz R, Imming P, Schulze W, Schumacher K, Rubinstein JL
Structure (2022) 30 pp. 1403- [ DOI: doi:10.1016/j.str.2022.07.006 Pubmed: 36041457 ]
- V-type proton atpase subunit c (16 kDa, Protein from Citrus limon)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Citrus limon)
- V-type proton atpase subunit g (12 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap2 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d (29 kDa, Protein from Citrus limon)
- V-type proton atpase subunit b2 (54 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c" (18 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e (26 kDa, Protein from Citrus limon)
- V-type proton atpase subunit e1 (7 kDa, Protein from Citrus limon)
- Citrus v-atpase (Complex from Citrus limon)
- V-type proton atpase subunit h (51 kDa, Protein from Citrus limon)
- V-type proton atpase subunit f (14 kDa, Protein from Citrus limon)
- V-type proton atpase subunit d2 (40 kDa, Protein from Citrus limon)
- V-type proton atpase subunit ap1 fragment (2 kDa, Protein from Citrus limon)
- V-type proton atpase subunit a3 (92 kDa, Protein from Citrus limon)
- V-type proton atpase subunit c (42 kDa, Protein from Citrus limon)