ClpP from Neisseria meningitidis - Compressed conformation
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7kr2
Q-score: 0.474
NmClpP compressed conformation
Ripstein ZA, Vahidi S, Rubinstein JL, Kay LE
J Am Chem Soc (2020) 142 pp. 20519-20523 [ Pubmed: 33232135 DOI: doi:10.1021/jacs.0c09474 ]
- Caseinolytic protease (316 kDa, Complex from Neisseria meningitidis)
ClpP1P2 complex from M. tuberculosis
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6vgk
Q-score: 0.491
Vahidi S, Ripstein ZA, Juravsky JB, Rennella E, Goldberg AL, Mittermaier AK, Rubinstein JL, Kay LE
PNAS (2020) 117 pp. 5895-5906 [ DOI: doi:10.1073/pnas.1921630117 Pubmed: 32123115 ]
- Atp-dependent clp protease proteolytic subunit 2 (21 kDa, Protein from Mycobacterium tuberculosis)
- Clpp1p2 complex (300 kDa, Complex from Mycobacterium tuberculosis)
- Atp-dependent clp protease proteolytic subunit 1 (21 kDa, Protein from Mycobacterium tuberculosis)
ClpP1P2 complex from M. tuberculosis bound to ADEP
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6vgn
Q-score: 0.494
Vahidi S, Ripstein ZA, Juravsky JB, Rennella E, Goldberg AL, Mittermaier AK, Rubinstein JL, Kay LE
PNAS (2020) 117 pp. 5895-5906 [ DOI: doi:10.1073/pnas.1921630117 Pubmed: 32123115 ]
- Atp-dependent clp protease proteolytic subunit (21 kDa, Protein from Mycobacterium tuberculosis)
- Atp-dependent clp protease proteolytic subunit 1 (21 kDa, Protein from Mycobacterium tuberculosis)
- R0m-wfp-alo-pro-ycp-ala-mp8 (800 Da, Protein from synthetic construct)
- Clpp1p2 complex with adep (300 kDa, Complex from Mycobacterium tuberculosis)
ClpP1P2 complex from M. tuberculosis with GLF-CMK bound to ClpP1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6vgq
Q-score: 0.459
Vahidi S, Ripstein ZA, Juravsky JB, Rennella E, Goldberg AL, Mittermaier AK, Rubinstein JL, Kay LE
PNAS (2020) 117 pp. 5895-5906 [ DOI: doi:10.1073/pnas.1921630117 Pubmed: 32123115 ]
- Atp-dependent clp protease proteolytic subunit (21 kDa, Protein from Mycobacterium tuberculosis)
- Clpp1p2 complex bound to glf-cmk (300 kDa, Complex from Mycobacterium tuberculosis)
- Z-gly-leu-phe-ch2cl (520 Da, Protein from synthetic construct)
- Atp-dependent clp protease proteolytic subunit 1 (21 kDa, Protein from Mycobacterium tuberculosis)
ClpXP from Neisseria meningitidis - Conformation A
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6vfs
Q-score: 0.301
Ripstein ZA, Vahidi S, Houry WA, Rubinstein JL, Kay LE
eLife (2020) 9 [ Pubmed: 31916936 DOI: doi:10.7554/eLife.52158 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpxp complex (860 kDa, Complex)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Neisseria meningitidis)
- Unidentified protein substrate (613 Da, Protein from Escherichia coli BL21(DE3))
- Un-identified protein substrate (Complex from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpx, atp-dependent clp protease proteolytic subunit (Complex from Neisseria meningitidis)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (45 kDa, Protein from Neisseria meningitidis)
ClpXP from Neisseria meningitidis - Conformation B
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6vfx
Q-score: 0.413
Ripstein ZA, Vahidi S, Houry WA, Rubinstein JL, Kay LE
eLife (2020) 9 [ Pubmed: 31916936 DOI: doi:10.7554/eLife.52158 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpxp complex (860 kDa, Complex)
- Unidentified peptide substrate (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Neisseria meningitidis)
- Atp-dependent clp protease atp-binding subunit clpx, atp-dependent clp protease proteolytic subunit (Complex from Neisseria meningitidis)
- Unidentified peptide substrate (613 Da, Protein from Escherichia coli BL21(DE3))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (45 kDa, Protein from Neisseria meningitidis)
ClpXP from N. meningitidis- D7 symmetry applied
Ripstein ZA, Vahidi S, Houry WA, Rubinstein JL, Kay LE
eLife (2020) 9 [ Pubmed: 31916936 DOI: doi:10.7554/eLife.52158 ]
- Clpxp complex (860 kDa, Complex from Neisseria meningitidis)
Ripstein ZA, Vahidi S, Houry WA, Rubinstein JL, Kay LE
eLife (2020) 9 [ Pubmed: 31916936 DOI: doi:10.7554/eLife.52158 ]
- Clpxp complex (860 kDa, Complex from Neisseria meningitidis)
cryo-EM density map of ClpP from Staphylococcus aureus (Wild Type)
Vahidi S, Ripstein ZA, Bonomi M, Yuwen T, Mabanglo MF, Juravsky JB, Rizzolo K, Velyvis A, Houry WA, Vendruscolo M, Rubinstein JL, Kay LE
PNAS (2018) 115 pp. E6447-E6456 [ DOI: doi:10.1073/pnas.1805125115 Pubmed: 29941580 ]