KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinement of KCTD5(CTD)/Gbeta1gamma2
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State A: Composite Map from RELION Multi-body Refinement
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8u81
Q-score: 0.228
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State A: Reference Map for Composite Map EMD-41996
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Kctd5 (Protein from Homo sapiens)
- Gbg2_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State B: Composite Map from RELION Multi-body Refinement
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 8u82
Q-score: 0.254
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State B: Reference Map for Composite Map EMD-42000
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State C: Composite Map from RELION Multi-body Refinement
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8u83
Q-score: 0.197
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
KCTD5/Cullin3/Gbeta1gamma2 Complex State D: Composite Map from RELION Multi-body Refinement
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 8u84
Q-score: 0.227
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
Structure of porcine V-ATPase with mEAK7 and SidK, Rotary state 2
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7u8o
Q-score: 0.371
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- Tldc protein meak-7 (50 kDa, Protein from Sus scrofa)
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
- V-atpase in complex with recombinant sidk and recombinant meak-7 (Complex from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 1
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7u8p
Q-score: 0.334
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Porcine kidney v-atpase with sidk, rotary state 1 (Complex from Sus scrofa)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)
Structure of porcine kidney V-ATPase with SidK, Rotary State 2
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7u8q
Q-score: 0.247
Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL
Life Sci Alliance (2022) 5 [ Pubmed: 35794005 DOI: doi:10.26508/lsa.202201527 ]
- V-type proton atpase proteolipid subunit (15 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit f (13 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit e 1 (26 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit d (28 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit a (96 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- V-type proton atpase subunit (40 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit c (44 kDa, Protein from Sus scrofa)
- Atpase h+ transporting accessory protein 1 (51 kDa, Protein from Sus scrofa)
- V-type proton atpase catalytic subunit a (68 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit g (13 kDa, Protein from Sus scrofa)
- Ribonuclease kappa (11 kDa, Protein from Sus scrofa)
- Bacterial effector protein sidk (38 kDa, Protein from Legionella pneumophila)
- V-type proton atpase 21 kda proteolipid subunit isoform 1 (21 kDa, Protein from Sus scrofa)
- Atpase h(+)-transporting lysosomal accessory protein 2 (39 kDa, Protein from Sus scrofa)
- Porcine kidney v-atpase with sidk, rotary state 2 (Complex from Sus scrofa)
- Vacuolar proton pump subunit b (57 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit (9 kDa, Protein from Sus scrofa)
- V-type proton atpase subunit h (55 kDa, Protein from Sus scrofa)