MPXV DNA polymerase complex in editing state 1
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 9k9u
Q-score: 0.364
Xie YF, Kuai L, Peng Q, Wang Q, Wang H, Li XM, Qi JX, Ding Q, Shi Y, Gao F
To Be Published
- E4r (25 kDa, Protein from Monkeypox virus)
- Dna (25-mer) (7 kDa, DNA from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna (38-mer) (11 kDa, DNA from Monkeypox virus)
- Dna polymerase (119 kDa, Protein from Monkeypox virus)
- Mpxv dna polymerase in complex with primer/4u template dna (Complex from Monkeypox virus)
The structure of ASFV DNA polymerase in replicating state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8ywi
Q-score: 0.508
Kuai L, Sun J, Peng Q, Zhao X, Yuan B, Liu S, Bi Y, Shi Y
Nucleic Acids Res (2024) 52 pp. 10717-10729 [ DOI: doi:10.1093/nar/gkae739 Pubmed: 39189451 ]
- The asfv dna polymerase in replicating state (Complex from African swine fever virus)
- The primer strand (7 kDa, DNA from African swine fever virus)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna polymerase (139 kDa, Protein from African swine fever virus)
- The template strand (11 kDa, DNA from African swine fever virus)
The structure of ASFV DNA polymerase in editing state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8ywm
Q-score: 0.346
Kuai L, Sun J, Peng Q, Zhao X, Yuan B, Liu S, Bi Y, Shi Y
Nucleic Acids Res (2024) 52 pp. 10717-10729 [ DOI: doi:10.1093/nar/gkae739 Pubmed: 39189451 ]
- Dna polymerase (139 kDa, Protein from African swine fever virus)
- The primer strand (6 kDa, DNA from African swine fever virus)
- The template strand (8 kDa, DNA from African swine fever virus)
- The asfv dna polymerase in editing state (Complex from African swine fever virus)
The structure of MPXV polymerase holoenzyme in replicating state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8hg1
Q-score: 0.486
Peng Q, Xie Y, Kuai L, Wang H, Qi J, Gao GF, Shi Y
Science (2023) 379 pp. 100-105 [ DOI: doi:10.1126/science.ade6360 Pubmed: 36520947 ]
- Dna (25-mer) (7 kDa, DNA from Monkeypox virus)
- Dna polymerase processivity factor component a20 (49 kDa, Protein from Monkeypox virus)
- Dna polymerase (119 kDa, Protein from Monkeypox virus)
- Mpxv dna polymerase holoenzyme (Complex from Monkeypox virus)
- Thymidine-5'-triphosphate (482 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Monkeypox virus)
- E4r (25 kDa, Protein from Monkeypox virus)
- Magnesium ion (24 Da, Ligand)
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 6m6a
Q-score: 0.256
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ DOI: doi:10.1093/nar/gkaa904 Pubmed: 33068413 ]
- Zinc ion (65 Da, Ligand)
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Transcription-repair-coupling factor (109 kDa, Protein from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Magnesium ion (24 Da, Ligand)
- Thermus thermophilus transcription-coupled dna repair complex (Complex from Thermus thermophilus)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6m6b
Q-score: 0.357
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ DOI: doi:10.1093/nar/gkaa904 Pubmed: 33068413 ]
- Zinc ion (65 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Transcription-repair-coupling factor (109 kDa, Protein from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039))
- Thermus thermophilus transcription-repair coupling complex (Complex from Thermus thermophilus)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
CryoEM structure of Thermus thermophilus RNA polymerase elongation complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6m6c
Q-score: 0.519
Shi J, Wen A, Zhao M, Jin S, You L, Shi Y, Dong S, Hua X, Zhang Y, Feng Y
Nucleic Acids Res (2020) 48 pp. 11762-11772 [ DOI: doi:10.1093/nar/gkaa904 Pubmed: 33068413 ]
- Rna (6 kDa, RNA from Thermus thermophilus)
- Zinc ion (65 Da, Ligand)
- Thermus thermophilus rna polymerase elongation complex (Complex from Thermus thermophilus)
- Nontemplate strand dna (19 kDa, DNA from Thermus thermophilus)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Dna-directed rna polymerase subunit beta' (170 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Template strand dna (19 kDa, DNA from Thermus thermophilus)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))