Structure of R2 with 3'UTR and DNA in binding state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ibw
Q-score: 0.385
Deng P, Tan SQ, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J, Liu JG
Cell (2023) 186 pp. 2865-2879.e20 [ DOI: doi:10.1016/j.cell.2023.05.032 Pubmed: 37301196 ]
- Reverse transcriptase-like protein (123 kDa, Protein from Bombyx mori)
- R2 complex (177 kDa, Complex from Bombyx mori)
- Dna (60-mer) (18 kDa, DNA from Bombyx mori)
- Zinc ion (65 Da, Ligand)
- 3'utr (16 kDa, RNA from Bombyx mori)
- Dna (60-mer) (18 kDa, DNA from Bombyx mori)
Structure of R2 with 3'UTR and DNA in unwinding state
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 8ibx
Q-score: 0.385
Deng P, Tan SQ, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J, Liu JG
Cell (2023) 186 pp. 2865-2879.e20 [ DOI: doi:10.1016/j.cell.2023.05.032 Pubmed: 37301196 ]
- Reverse transcriptase-like protein (123 kDa, Protein from Bombyx mori)
- R2 complex (177 kDa, Complex from Bombyx mori)
- Dna (60-mer) (18 kDa, DNA from Bombyx mori)
- Zinc ion (65 Da, Ligand)
- 3'utr (16 kDa, RNA from Bombyx mori)
- Dna (60-mer) (18 kDa, DNA from Bombyx mori)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 8iby
Q-score: 0.342
Deng P, Tan SQ, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J, Liu JG
Cell (2023) 186 pp. 2865-2879.e20 [ DOI: doi:10.1016/j.cell.2023.05.032 Pubmed: 37301196 ]
- Reverse transcriptase-like protein (123 kDa, Protein from Bombyx mori)
- 5'orf rna (72 kDa, RNA from Bombyx mori)
- R2 complex (177 kDa, Complex from Bombyx mori)
- Zinc ion (65 Da, Ligand)
Structure of R2 with 5'ORF and 3'UTR
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 8ibz
Q-score: 0.435
Deng P, Tan SQ, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J, Liu JG
Cell (2023) 186 pp. 2865-2879.e20 [ DOI: doi:10.1016/j.cell.2023.05.032 Pubmed: 37301196 ]
- Reverse transcriptase-like protein (123 kDa, Protein from Bombyx mori)
- Zinc ion (65 Da, Ligand)
- R2 complex (177 kDa, Complex from Bombyx mori)
- 5orf-linker-3utr (65 kDa, RNA from Bombyx mori)
In situ structure of polymerase complex of mammalian reovirus in the elongation state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7yed
Q-score: 0.498
Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P
PNAS (2022) 119 pp. e2203054119-e2203054119 [ DOI: doi:10.1073/pnas.2203054119 Pubmed: 36469786 ]
- Magnesium ion (24 Da, Ligand)
- Mammalian orthoreovirus 3 (Complex)
- Rna-directed rna polymerase (142 kDa, Protein from Mammalian orthoreovirus 3)
- Rna (5'-r(p*up*up*up*ap*ap*ap*ap*ap*up*up*up*up*ap*ap*ap*ap*up*ap*ap*up*u)-3') (6 kDa, RNA from Mammalian orthoreovirus 3)
- Rna helicase (141 kDa, Protein from Mammalian orthoreovirus 3)
- Lambda-2 protein (143 kDa, Protein from Mammalian orthoreovirus 3)
- Rna (36-mer) (11 kDa, RNA from Mammalian orthoreovirus 3)
- Zinc ion (65 Da, Ligand)
- S-adenosylmethionine (398 Da, Ligand)
- Mammalian orthoreovirus 3 (Virus from Mammalian orthoreovirus 3)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Mu-2 protein (83 kDa, Protein from Mammalian orthoreovirus 3)
- Uridine 5'-triphosphate (484 Da, Ligand)
- Rna (Complex)
In situ structure of polymerase complex of mammalian reovirus in the pre-elongation state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7yev
Q-score: 0.457
Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P
PNAS (2022) 119 pp. e2203054119-e2203054119 [ DOI: doi:10.1073/pnas.2203054119 Pubmed: 36469786 ]
- Rna-directed rna polymerase (142 kDa, Protein from Mammalian orthoreovirus 3)
- S-adenosylmethionine (398 Da, Ligand)
- Rna helicase (141 kDa, Protein from Mammalian orthoreovirus 3)
- Lambda-2 protein (143 kDa, Protein from Mammalian orthoreovirus 3)
- Zinc ion (65 Da, Ligand)
- Mammalian orthoreovirus 3 (Virus from Mammalian orthoreovirus 3)
- Mu-2 protein (83 kDa, Protein from Mammalian orthoreovirus 3)
In situ structure of polymerase complex of mammalian reovirus in the reloaded state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yez
Q-score: 0.384
Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P
PNAS (2022) 119 pp. e2203054119-e2203054119 [ DOI: doi:10.1073/pnas.2203054119 Pubmed: 36469786 ]
- Rna-directed rna polymerase (142 kDa, Protein from Mammalian orthoreovirus 3)
- S-adenosylmethionine (398 Da, Ligand)
- Rna helicase (141 kDa, Protein from Mammalian orthoreovirus 3)
- Lambda-2 protein (143 kDa, Protein from Mammalian orthoreovirus 3)
- Zinc ion (65 Da, Ligand)
- Mammalian orthoreovirus 3 (Virus from Mammalian orthoreovirus 3)
- Mu-2 protein (83 kDa, Protein from Mammalian orthoreovirus 3)
In situ structure of polymerase complex of mammalian reovirus in the core
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yf0
Q-score: 0.441
Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P
PNAS (2022) 119 pp. e2203054119-e2203054119 [ DOI: doi:10.1073/pnas.2203054119 Pubmed: 36469786 ]
- Rna-directed rna polymerase (142 kDa, Protein from Mammalian orthoreovirus 3)
- Rna helicase (141 kDa, Protein from Mammalian orthoreovirus 3)
- Lambda-2 protein (143 kDa, Protein from Mammalian orthoreovirus 3)
- Zinc ion (65 Da, Ligand)
- Mammalian orthoreovirus 3 (Virus from Mammalian orthoreovirus 3)
- Mu-2 protein (83 kDa, Protein from Mammalian orthoreovirus 3)
In situ structure of polymerase complex of mammalian reovirus in virion
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yfe
Q-score: 0.422
Bao K, Zhang X, Li D, Sun W, Sun Z, Wang J, Zhu P
PNAS (2022) 119 pp. e2203054119-e2203054119 [ DOI: doi:10.1073/pnas.2203054119 Pubmed: 36469786 ]
- Rna (5'-r(p*gp*cp*u)-3') (911 Da, RNA from Mammalian orthoreovirus 3)
- Rna-directed rna polymerase (142 kDa, Protein from Mammalian orthoreovirus 3)
- Rna helicase (141 kDa, Protein from Mammalian orthoreovirus 3)
- Lambda-2 protein (143 kDa, Protein from Mammalian orthoreovirus 3)
- Rna (5'-r(p*ap*cp*gp*ap*up*up*ap*gp*c)-3') (2 kDa, RNA from Mammalian orthoreovirus 3)
- Zinc ion (65 Da, Ligand)
- Mammalian orthoreovirus 3 (Virus)
- Mu-2 protein (83 kDa, Protein from Mammalian orthoreovirus 3)
- Mammalian orthoreovirus 3 (Complex from Mammalian orthoreovirus 3)
- Rna (5'-r(p*ap*up*cp*gp*u)-3') (1 kDa, RNA from Mammalian orthoreovirus 3)
- Rna (Complex)