Cryo-EM structure of SaCas9-AcrIIA15 CTD-sgRNA complex
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 8jft
Q-score: 0.426
Deng X, Sun W, Li X, Wang J, Cheng Z, Sheng G, Wang Y
Nat Commun (2024) 15 pp. 1806-1806 [ Pubmed: 38418450 DOI: doi:10.1038/s41467-024-45987-5 ]
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Staphylococcus aureus)
- Ternary complex of sacas9-acriia15 ctd-sgrna complex (Complex)
- Sacas9 (Complex from Staphylococcus aureus)
- Acriia15 (13 kDa, Protein from Staphylococcus delphini)
- Sgrna of sacas9 (31 kDa, RNA from Staphylococcus aureus)
- Acriia15 (Complex from Staphylococcus delphini)
- Sgrna (Complex from Staphylococcus aureus)
Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8jg9
Q-score: 0.173
Deng X, Sun W, Li X, Wang J, Cheng Z, Sheng G, Wang Y
Nat Commun (2024) 15 pp. 1806-1806 [ Pubmed: 38418450 DOI: doi:10.1038/s41467-024-45987-5 ]
- Acriia15 (20 kDa, Protein from Staphylococcus delphini)
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Staphylococcus aureus)
- Dna (Complex)
- Sgrna (Complex from Staphylococcus delphini)
- Sacas9 (Complex)
- Dna (25-mer) (7 kDa, DNA from Staphylococcus delphini)
- Sacas9-sgrna-acriia15-promoter dna complex (Complex from Staphylococcus aureus)
- Acriia15 (Complex)
- Sgrna (31 kDa, RNA from Staphylococcus aureus)
- Dna (25-mer) (7 kDa, DNA from Staphylococcus delphini)
Local refined cryo-EM reconstruction of Omicron BA.5 RBD in complex with 8-9D Fab
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8j1t
Q-score: 0.455
Tai W, Yang K, Liu Y, Li R, Feng S, Chai B, Zhuang X, Qi S, Shi H, Liu Z, Lei J, Ma E, Wang W, Tian C, Le T, Wang J, Chen Y, Tian M, Xiang Y, Yu G, Cheng G
Nat Commun (2023) 14 pp. 8042-8042 [ DOI: doi:10.1038/s41467-023-43798-8 Pubmed: 38052844 ]
- Spike protein (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Severe acute respiratory syndrome coronavirus 2 omicron ba.5 rbd bound 8-9d fab (22 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov2 omicron ba.5 rbd (Complex)
- 8-9d heavy chain (12 kDa, Protein from Homo sapiens)
- 8-9d light chain (11 kDa, Protein from Homo sapiens)
- 8-9d fab (Complex from Homo sapiens)
Cryo-EM reconstruction of SARS-CoV2 Omicron BA.5 spike in complex with 8-9D Fabs
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8j1v
Q-score: 0.374
Tai W, Yang K, Liu Y, Li R, Feng S, Chai B, Zhuang X, Qi S, Shi H, Liu Z, Lei J, Ma E, Wang W, Tian C, Le T, Wang J, Chen Y, Tian M, Xiang Y, Yu G, Cheng G
Nat Commun (2023) 14 pp. 8042-8042 [ DOI: doi:10.1038/s41467-023-43798-8 Pubmed: 38052844 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sars-cov2 omicron ba.5 spike ectodomain (Complex)
- 8-9d heavy chain (12 kDa, Protein from Homo sapiens)
- Severe acute respiratory syndrome coronavirus 2 omicron ba.5 spike bound 8-9d fab (369 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- 8-9d fab variable region (Complex from Homo sapiens)
- 8-9d light chain (11 kDa, Protein from Homo sapiens)
- Spike protein s2' (123 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8hnv
Q-score: 0.464
Sun W, Cheng Z, Wang J, Yang J, Li X, Wang J, Chen M, Yang X, Sheng G, Lou J, Wang Y
PNAS (2023) 120 pp. e2303675120-e2303675120 [ DOI: doi:10.1073/pnas.2303675120 Pubmed: 37494395 ]
- Complex of hpacas9-sgrna-dna in the presence of acriic4 (194 kDa, Complex from Haemophilus parainfluenzae)
- Non-target strand (10 kDa, DNA from Haemophilus parainfluenzae)
- Rna/dna (Complex)
- Target strand (10 kDa, DNA from Haemophilus parainfluenzae)
- Anti-crispr protein acriic4 (10 kDa, Protein from Haemophilus parainfluenzae)
- Hpacas9/acriic4 (Complex)
- Sgrna (40 kDa, RNA from synthetic construct)
- Crispr-associated endonuclease cas9 (121 kDa, Protein from Haemophilus parainfluenzae)
Cryo-EM structure of ABC transporter ABCC3
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8hvh
Q-score: 0.431
Wang J, Li X, Wang FF, Cheng MT, Mao YX, Fang SC, Wang L, Zhou CZ, Hou WT, Chen Y
EMBO J (2023) 42 pp. e113415-e113415 [ Pubmed: 37485728 DOI: doi:10.15252/embj.2022113415 ]
- Human abc transporter abcc3 in nanodiscs (169 MDa, Complex from Homo sapiens)
- Atp-binding cassette sub-family c member 3 (169 kDa, Protein from Homo sapiens)
Cryo-EM structure of dehydroepiandrosterone sulfate-bound human ABC transporter ABCC3 in nanodiscs
Cryo-EM structure of the gasdermin B pore
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8gtn
Q-score: 0.421
Zhong X, Zeng H, Zhou Z, Su Y, Cheng H, Hou Y, She Y, Feng N, Wang J, Shao F, Ding J
Nature (2023) 616 pp. 598-605 [ DOI: doi:10.1038/s41586-023-05872-5 Pubmed: 36991125 ]
- Pore-forming protein gasdermin b (Complex from Homo sapiens)
- Isoform 4 of gasdermin-b (28 kDa, Protein from Homo sapiens)
CryoEM structure of an anti-CRISPR protein AcrIIC5 bound to Nme1Cas9-sgRNA complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8hj4
Q-score: 0.425
Sun W, Zhao X, Wang J, Yang X, Cheng Z, Liu S, Wang J, Sheng G, Wang Y
Nucleic Acids Res (2023) 51 pp. 1984-1995 [ DOI: doi:10.1093/nar/gkad052 Pubmed: 36744495 ]
- Complex of acriic5 with sgrna-bound nme1cas9 (183 MDa, Complex from Neisseria meningitidis 8013)
- Sgrna (43 kDa, RNA from Neisseria meningitidis 8013)
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Neisseria meningitidis serogroup C (strain 8013))
- Phage protein (15 kDa, Protein from Simonsiella muelleri ATCC 29453)