Cryo-EM structure of the xGPR4-Gs complex in pH6.2
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8zd1
Q-score: 0.567
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cryo-em structure of the xgpr4-gs complex in ph6.2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (5 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (31 kDa, Protein from Xenopus tropicalis)
Cryo-EM structure of the receptor of xGPR4-Gs complex in pH6.2
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 8zf4
Q-score: 0.497
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
Cryo-EM structure of the xGPR4-Gs complex in pH6.7
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8zf6
Q-score: 0.53
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Cryo-em structure of the xgpr4-gs complex in ph6.7 (Complex from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the receptor of xGPR4-Gs complex in pH6.7
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8zf7
Q-score: 0.495
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
Cryo-EM structure of the xtGPR4-Gs complex in pH7.2
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8zfa
Q-score: 0.483
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
- Cryo-em structure of the xtgpr4-gs complex in ph7.2 (Complex from Xenopus tropicalis)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- G-protein coupled receptor 4 (38 kDa, Protein from Xenopus tropicalis)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
Cryo-EM structure of the receptor of xtGPR4-Gs complex in pH7.2
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 8zfb
Q-score: 0.433
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
Cryo-EM structure of the receptor of xGPR4-apo in pH8.0
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 9jvm
Q-score: 0.393
Wen X, Shang P, Chen H, Guo L, Rong N, Jiang X, Li X, Liu J, Yang G, Zhang J, Zhu K, Meng Q, He X, Wang Z, Liu Z, Cheng H, Zheng Y, Zhang B, Pang J, Liu Z, Xiao P, Chen Y, Liu L, Luo F, Yu X, Yi F, Zhang P, Yang F, Deng C, Sun JP
Cell (2025) 188 pp. 653-670.e24 [ Pubmed: 39753131 DOI: doi:10.1016/j.cell.2024.12.001 ]
NSD2 bearing E1099K/T1150A dual mutation in complex with 187-bp NCP
Resolution: 3.75 Å
EM Method: Single-particle
Fitted PDBs: 7cro
Q-score: 0.448
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Nsd2 (Complex from Homo sapiens)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase nsd2 (79 kDa, Protein from Homo sapiens)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Histone h3, h4, h2a and h2b (Complex from Xenopus laevis)
- Dna (Complex from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Nsd2 bearing e1099k/t1150a dual mutation in complex with 187-bp ncp (300 kDa, Complex)
NSD3 bearing E1181K/T1232A dual mutation in complex with 187-bp NCP (1:1 binding mode)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7crp
Q-score: 0.542
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Nsd3 bearing e1181k/t1232a dual mutation in complex with 187-bp ncp (1:1 binding mode) (300 kDa, Complex)
- Nsd3 (Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Histone h3, h4, h2a, h2b (Complex from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase nsd3 (85 kDa, Protein from Homo sapiens)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Dna (Complex from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
NSD3 bearing E1181K/T1232A dual mutation in complex with 187-bp NCP (2:1 binding mode)
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7crq
Q-score: 0.507
Li W, Tian W, Yuan G, Deng P, Sengupta D, Cheng Z, Cao Y, Ren J, Qin Y, Zhou Y, Jia Y, Gozani O, Patel DJ, Wang Z
Nature (2021) 590 pp. 498-503 [ Pubmed: 33361816 DOI: doi:10.1038/s41586-020-03069-8 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Nsd3 (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histone h3, h4, h2a, h2b (Complex from Xenopus laevis)
- S-adenosylmethionine (398 Da, Ligand)
- Histone-lysine n-methyltransferase nsd3 (85 kDa, Protein from Homo sapiens)
- Histone h2a (13 kDa, Protein from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Dna (Complex from Xenopus laevis)
- Dna (168-mer) (57 kDa, DNA from Xenopus laevis)
- Histone h2b (13 kDa, Protein from Xenopus tropicalis)
- Histone h3 (15 kDa, Protein from Xenopus laevis)
- Nsd3 bearing e1181k/t1232a dual mutation in complex with 187-bp ncp (2:1 binding mode) (300 kDa, Complex)