Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 9chu
Q-score: 0.478
Xu J, Chen G, Wang H, Cao S, Heng J, Deupi X, Du Y, Kobilka BK
PNAS (2024) 121 pp. e2414544121-e2414544121 [ DOI: doi:10.1073/pnas.2414544121 Pubmed: 39565314 ]
- Fkbp1a (Complex from Homo sapiens)
- Calcineurin fused beta2ar (Complex from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (42 kDa, Protein from Mus musculus)
- 8-deethyl-8-[but-3-enyl]-ascomycin (804 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1a (11 kDa, Protein from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (Complex from Mus musculus)
- Calcineurin fused beta2ar in complex with fkbp12 (Complex)
- Beta-2 adrenergic receptor,calcineurin subunit b type 1 (49 kDa, Protein from Homo sapiens)
- Noradrenaline (170 Da, Ligand)
cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in apo state
Resolution: 3.95 Å
EM Method: Single-particle
Fitted PDBs: 9chv
Q-score: 0.39
Xu J, Chen G, Wang H, Cao S, Heng J, Deupi X, Du Y, Kobilka BK
PNAS (2024) 121 pp. e2414544121-e2414544121 [ DOI: doi:10.1073/pnas.2414544121 Pubmed: 39565314 ]
- Fkbp1a (Complex from Homo sapiens)
- Calcineurin fused beta2ar (Complex from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (42 kDa, Protein from Mus musculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1a (11 kDa, Protein from Homo sapiens)
- Beta-2 adrenergic receptor,calcineurin subunit b type 1 (51 kDa, Protein from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (Complex from Mus musculus)
- Calcineurin fused beta2ar in complex with fkbp12 (Complex)
- 8-deethyl-8-[but-3-enyl]-ascomycin (804 Da, Ligand)
cryo-EM structure of calcineurin-fused beta2 adrenergic receptor in carazolol bound inactive state
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9chx
Q-score: 0.431
Xu J, Chen G, Wang H, Cao S, Heng J, Deupi X, Du Y, Kobilka BK
PNAS (2024) 121 pp. e2414544121-e2414544121 [ DOI: doi:10.1073/pnas.2414544121 Pubmed: 39565314 ]
- Fkbp1a (Complex from Homo sapiens)
- Calcineurin fused beta2ar (Complex from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (42 kDa, Protein from Mus musculus)
- (2s)-1-(9h-carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol (298 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1a (11 kDa, Protein from Homo sapiens)
- Beta-2 adrenergic receptor,calcineurin subunit b type 1 (51 kDa, Protein from Homo sapiens)
- Protein phosphatase 3 catalytic subunit alpha (Complex from Mus musculus)
- Calcineurin fused beta2ar in complex with fkbp12 (Complex)
- 8-deethyl-8-[but-3-enyl]-ascomycin (804 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8t6j
Q-score: 0.463
Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK
Nature (2024) 629 pp. 951-956 [ DOI: doi:10.1038/s41586-024-07327-x Pubmed: 38632403 ]
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8t7h
Q-score: 0.43
Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK
Nature (2024) 629 pp. 951-956 [ DOI: doi:10.1038/s41586-024-07327-x Pubmed: 38632403 ]
- Nanobody 43 (13 kDa, Protein from Lama glama)
- Metabotropic glutamate receptor 5 in complex with quis (Cellular component from Homo sapiens)
- (s)-2-amino-3-(3,5-dioxo-[1,2,4]oxadiazolidin-2-yl)-propionic acid (189 Da, Ligand)
- Metabotropic glutamate receptor 5 (98 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8t8m
Q-score: 0.493
Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK
Nature (2024) 629 pp. 951-956 [ DOI: doi:10.1038/s41586-024-07327-x Pubmed: 38632403 ]
- Nb43 (13 kDa, Protein from Lama glama)
- Metabotropic glutamate receptor 5 in complex with quis (Cellular component from Homo sapiens)
- (s)-2-amino-3-(3,5-dioxo-[1,2,4]oxadiazolidin-2-yl)-propionic acid (189 Da, Ligand)
- Metabotropic glutamate receptor 5 (98 kDa, Protein from Homo sapiens)
Quis and CDPPB bound active mGlu5
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8tao
Q-score: 0.486
Krishna Kumar K, Wang H, Habrian C, Latorraca NR, Xu J, O'Brien ES, Zhang C, Montabana E, Koehl A, Marqusee S, Isacoff EY, Kobilka BK
Nature (2024) 629 pp. 951-956 [ DOI: doi:10.1038/s41586-024-07327-x Pubmed: 38632403 ]
- Nb43 (13 kDa, Protein from Lama glama)
- 3-cyano-n-(1,3-diphenyl-1h-pyrazol-5-yl)benzamide (364 Da, Ligand)
- Metabotropic glutamate receptor 5 bound to quis and cdppb, in complex with nb43 (Cellular component from Homo sapiens)
- Metabotropic glutamate receptor 5 (98 kDa, Protein from Homo sapiens)
- (s)-2-amino-3-(3,5-dioxo-[1,2,4]oxadiazolidin-2-yl)-propionic acid (189 Da, Ligand)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7t8x
Q-score: 0.517
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7t90
Q-score: 0.47
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7t94
Q-score: 0.46
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)