Yeast tubulin polymerized with GTP in vitro
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3f
Q-score: 0.366
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynamic microtubule lattice (Complex from Saccharomyces cerevisiae S288c)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with epothilone
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3h
Q-score: 0.369
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Epothilone a (493 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Microtubule lattice stabilized with epothilone (Complex from Saccharomyces cerevisiae S288c)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule stabilized with Taxol assembled from mutated tubulin
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 5w3j
Q-score: 0.338
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Yeast microtubule stabilized with taxol assembled from mutated tubulin (Complex from Saccharomyces cerevisiae S288c)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin beta chain (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast microtubule assembled using the slowly hydrolyzable analogue GMPCPP
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
Yeast microtubule assembled using the slowly hydrolyzable analogue GTPgammaS
Howes SC, Geyer EA, LaFrance B, Zhang R, Kellogg EH, Westermann S, Rice LM, Nogales E
J Cell Biol (2017) 216 pp. 2669-2677 [ Pubmed: 28652389 DOI: doi:10.1083/jcb.201612195 ]
Cryo EM density of microtubules stabilized by GMPCPP
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 3j6e
Q-score: 0.243
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell (2014) 157 pp. 1117-1129 [ Pubmed: 24855948 DOI: doi:10.1016/j.cell.2014.03.053 ]
- Beta tubulin (55 kDa, Protein from Sus scrofa)
- Alpha tubulin (55 kDa, Protein from Sus scrofa)
- Kinesin (36 kDa, Protein from Homo sapiens)
- Microtubule stabilized by gmpcpp (Sample)
Cryo EM density of GDP-bound dynamic microtubules
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 3j6f
Q-score: 0.222
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell (2014) 157 pp. 1117-1129 [ Pubmed: 24855948 DOI: doi:10.1016/j.cell.2014.03.053 ]
- Beta tubulin (55 kDa, Protein from Sus scrofa)
- Alpha tubulin (55 kDa, Protein from Sus scrofa)
- Kinesin (36 kDa, Protein from Homo sapiens)
- Dynamic gdp-bound microtubule (Sample)
Cryo EM density of microtubules stabilized by taxol
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 3j6g
Q-score: 0.194
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell (2014) 157 pp. 1117-1129 [ Pubmed: 24855948 DOI: doi:10.1016/j.cell.2014.03.053 ]
- Beta tubulin (55 kDa, Protein from Sus scrofa)
- Alpha tubulin (55 kDa, Protein from Sus scrofa)
- Kinesin (36 kDa, Protein from Homo sapiens)
- Microtubule stabilized by taxol (Sample)
Cryo EM density of microtubules stabilized by GMPCPP, without kinesin
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell (2014) 157 pp. 1117-1129 [ Pubmed: 24855948 DOI: doi:10.1016/j.cell.2014.03.053 ]
- Beta tubulin (55 kDa, Protein from Sus scrofa)
- Alpha tubulin (55 kDa, Protein from Sus scrofa)
- Microtubule stabilized by gmpcpp, without kinesin (Sample)
Cryo EM density of microtubules bound to GDP, no kinesin
Alushin GM, Lander GC, Kellogg EH, Zhang R, Baker D, Nogales E
Cell (2014) 157 pp. 1117-1129 [ Pubmed: 24855948 DOI: doi:10.1016/j.cell.2014.03.053 ]
- Beta tubulin (55 kDa, Protein from Sus scrofa)
- Alpha tubulin (55 kDa, Protein from Sus scrofa)
- Dynamic microtubule bound to gdp, without kinesin (Sample)