Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7t8x
Q-score: 0.517
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7t90
Q-score: 0.47
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7t94
Q-score: 0.46
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of S2 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7t96
Q-score: 0.466
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
PZM21 bound Mu Opioid Receptor-Gi Protein Complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7sbf
Q-score: 0.541
Wang H, Hetzer F, Huang W, Qu Q, Meyerowitz J, Kaindl J, Hubner H, Skiniotis G, Kobilka BK, Gmeiner P
Angew Chem Int Ed Engl (2022) 61 pp. e202200269-e202200269 [ Pubmed: 35385593 DOI: doi:10.1002/anie.202200269 ]
- N-[(2s)-2-(dimethylamino)-3-(4-hydroxyphenyl)propyl]-n'-[(2s)-1-(thiophen-3-yl)propan-2-yl]urea (361 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Pzm21 bound mu opioid receptor-gi protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562, mu-type opioid receptor chimera,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
FH210 bound Mu Opioid Receptor-Gi Protein Complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7scg
Q-score: 0.575
Wang H, Hetzer F, Huang W, Qu Q, Meyerowitz J, Kaindl J, Hubner H, Skiniotis G, Kobilka BK, Gmeiner P
Angew Chem Int Ed Engl (2022) 61 pp. e202200269-e202200269 [ Pubmed: 35385593 DOI: doi:10.1002/anie.202200269 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (2e)-n-[(2s)-2-(dimethylamino)-3-(4-hydroxyphenyl)propyl]-3-(naphthalen-1-yl)prop-2-enamide (374 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- Fh210 bound mu opioid receptor-gi protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Scfv16 (27 kDa, Protein from Mus musculus)