CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t2g
Q-score: 0.672
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor / gi protein complex bound to mitragynine pseudoindoxyl (mp). (Complex from Mus musculus)
- Mitragynine pseudoindoxyl (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2h
Q-score: 0.528
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Lofentanil (408 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor - gi protein complex bound to lofentanil (lft) (Complex from Mus musculus)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
C6-guano bound Mu Opioid Receptor-Gi Protein Complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7u2k
Q-score: 0.527
Faouzi A, Wang H, Zaidi SA, DiBerto JF, Che T, Qu Q, Robertson MJ, Madasu MK, El Daibani A, Varga BR, Zhang T, Ruiz C, Liu S, Xu J, Appourchaux K, Slocum ST, Eans SO, Cameron MD, Al-Hasani R, Pan YX, Roth BL, McLaughlin JP, Skiniotis G, Katritch V, Kobilka BK, Majumdar S
Nature (2023) 613 pp. 767-774 [ Pubmed: 36450356 DOI: doi:10.1038/s41586-022-05588-y ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- C6guano bound mu opioid receptor-gi protein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- N-(6-carbamimidamidohexyl)-n-[1-(2-phenylethyl)piperidin-4-yl]propanamide (401 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7ran
Q-score: 0.49
Kaplan AL, Confair DN, Kim K, Barros-Alvarez X, Rodriguiz RM, Yang Y, Kweon OS, Che T, McCorvy JD, Kamber DN, Phelan JP, Martins LC, Pogorelov VM, DiBerto JF, Slocum ST, Huang XP, Kumar JM, Robertson MJ, Panova O, Seven AB, Wetsel AQ, Wetsel WC, Irwin JJ, Skiniotis G, Shoichet BK, Roth BL, Ellman JA
Nature (2022) 610 pp. 582-591 [ DOI: doi:10.1038/s41586-022-05258-z Pubmed: 36171289 ]
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- (3r)-3-methyl-5-(1h-pyrrolo[2,3-b]pyridin-3-yl)-1,2,3,6-tetrahydropyridin-1-ium (214 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 5-ht2ar bound to a tetrahydropyridine agonist (r-69) in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ul2
Q-score: 0.645
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Ntsr1 (Complex from Homo sapiens)
- Neurotensin receptor 1 (47 kDa, Protein from Homo sapiens)
- Complex of ntsr1 and nb6 (Complex from Homo sapiens)
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Sodium ion (22 Da, Ligand)
- Nb6 (Complex from Lama glama)
- 2-[[1-(7-chloranylquinolin-4-yl)-5-(2,6-dimethoxyphenyl)pyrazol-3-yl]carbonylamino]adamantane-2-carboxylic acid (587 Da, Ligand)
- Water (18 Da, Ligand)
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- H2r (Complex from Homo sapiens)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Famotidine (339 Da, Ligand)
- Nb6m (Complex from Lama glama)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab (Complex from synthetic construct)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ul4
Q-score: 0.563
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Complex of mor and nb6 (Complex from Mus musculus)
- Megabody 6 (55 kDa, Protein from synthetic construct)
- N-[(2s)-2-{[(3r,4r)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine (424 Da, Ligand)
- Mb6 (Complex from Lama glama)
- Mor (Complex from Mus musculus)
- Mu-type opioid receptor (47 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
CryoEM Structure of Inactive SSTR2 bound to Nb6
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ul5
Q-score: 0.562
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Somatostatin receptor type 2 (46 kDa, Protein from Homo sapiens)
- Complex of sstr2 and nb6 (Complex from Homo sapiens)
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Sstr2 (Complex from Homo sapiens)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7u2l
Q-score: 0.525
Faouzi A, Wang H, Zaidi SA, DiBerto JF, Che T, Qu Q, Robertson MJ, Madasu MK, El Daibani A, Varga BR, Zhang T, Ruiz C, Liu S, Xu J, Appourchaux K, Slocum ST, Eans SO, Cameron MD, Al-Hasani R, Pan YX, Roth BL, McLaughlin JP, Skiniotis G, Katritch V, Kobilka BK, Majumdar S
Nature (2023) 613 pp. 767-774 [ Pubmed: 36450356 DOI: doi:10.1038/s41586-022-05588-y ]
- C5guano-uor-gi-scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- N-(5-carbamimidamidopentyl)-n-[1-(2-phenylethyl)piperidin-4-yl]propanamide (387 Da, Ligand)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
Apo form metabotropic glutamate receptor 5 with Nanobody 43
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laermans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature (2019) 566 pp. 79-84 [ DOI: doi:10.1038/s41586-019-0881-4 Pubmed: 30675062 ]
- Nanobody 43 (Protein from Lama glama)
- Metabotropic glutamate receptor 5 - nb43 complex (220 kDa, Complex from Homo sapiens)
- Metabotropic glutamate receptor 5 (Protein from Homo sapiens)