Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025) [ Pubmed: 39978345 DOI: doi:10.1016/j.cell.2025.01.037 ]
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Substrate ftsq-lacy(+1c) (7 kDa, Protein from Escherichia coli K-12)
- Seca-secy complex with the substrate ftsq-lacy(+1c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025) [ Pubmed: 39978345 DOI: doi:10.1016/j.cell.2025.01.037 ]
- Magnesium ion (24 Da, Ligand)
- Protein translocase subunit sece (6 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Nanobody (12 kDa, Protein from Lama glama)
- Seca-secy complex with the substrate ftsq-lacy(+7c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (7 kDa, Protein from Escherichia coli K-12)
- Nanobody (12 kDa, Protein from Lama glama)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Protein translocase subunit seca (87 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+20C)
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025) [ Pubmed: 39978345 DOI: doi:10.1016/j.cell.2025.01.037 ]
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit seca (88 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Magnesium ion (24 Da, Ligand)
- Green fluorescent protein (25 kDa, Protein from Aequorea victoria)
- Seca-secy complex with the substrate ftsq-lacy(+20c) (Complex from Geobacillus thermodenitrificans NG80-2)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Nanobody (12 kDa, Protein from Lama glama)
- Beryllium trifluoride ion (66 Da, Ligand)
Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C) treated with DTT
Ou X, Ma C, Sun D, Xu J, Wang Y, Wu X, Wang D, Yang S, Gao N, Song C, Li L
Cell (2025) [ Pubmed: 39978345 DOI: doi:10.1016/j.cell.2025.01.037 ]
- Protein translocase subunit sece (8 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Protein translocase subunit secy (47 kDa, Protein from Geobacillus thermodenitrificans NG80-2)
- Cell division protein ftsq,lactose permease (8 kDa, Protein from Escherichia coli K-12)
- Seca-secy complex with the substrate ftsq-lacy(+7c) treated with dtt (Complex from Geobacillus thermodenitrificans NG80-2)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8zmr
Q-score: 0.393
Zhang Y, Dai F, Chen N, Zhou D, Lee CH, Song C, Zhang Y, Zhang Z
Cell Res (2024) 34 pp. 665-668 [ DOI: doi:10.1038/s41422-024-00986-5 Pubmed: 38862671 ]
- Vacht (60 kDa, Complex from Escherichia coli (strain K12))
- Vesamicol (259 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,vesicular acetylcholine transporter,darpinoff7 (112 kDa, Protein from synthetic construct)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8zms
Q-score: 0.407
Zhang Y, Dai F, Chen N, Zhou D, Lee CH, Song C, Zhang Y, Zhang Z
Cell Res (2024) 34 pp. 665-668 [ DOI: doi:10.1038/s41422-024-00986-5 Pubmed: 38862671 ]
- Vacht (60 kDa, Complex from Escherichia coli (strain K12))
- Acetylcholine (146 Da, Ligand)
- Maltose/maltodextrin-binding periplasmic protein,vesicular acetylcholine transporter,darpinoff7 (112 kDa, Protein from synthetic construct)
Structure of divisome complex FtsWIQLB
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8p1u
Q-score: 0.45
Yang L, Chen Y, Chang S, Shen C, Wang X, Zhang C, Zhang Z, Ding BS, Su Z, Dong H, Tang X
Cell Discov (2024) 10 pp. 2-2 [ Pubmed: 38172099 DOI: doi:10.1038/s41421-023-00629-w ]
- Cell division protein ftsq (32 kDa, Protein from Pseudomonas aeruginosa)
- Cell division protein ftsl (11 kDa, Protein from Pseudomonas aeruginosa)
- Divisome complex ftswiqlb (Complex from Escherichia coli K-12)
- Peptidoglycan d,d-transpeptidase ftsi (62 kDa, Protein from Pseudomonas aeruginosa)
- Cell division protein ftsb (10 kDa, Protein from Pseudomonas aeruginosa)
- Probable peptidoglycan glycosyltransferase ftsw (43 kDa, Protein from Pseudomonas aeruginosa)
Wee LM, Tong AB, Florez Ariza AJ, Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ
Cell (2023) 186 pp. 1244-1262.e34 [ Pubmed: 36931247 DOI: doi:10.1016/j.cell.2023.02.008 ]
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8fix
Q-score: 0.3
Wee LM, Tong AB, Florez Ariza AJ, Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ
Cell (2023) 186 pp. 1244-1262.e34 [ Pubmed: 36931247 DOI: doi:10.1016/j.cell.2023.02.008 ]
- Rna (3 kDa, RNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- E. coli rna polymerase complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna (Complex from Escherichia phage Lambda)
- Template dna (7 kDa, DNA from Escherichia phage Lambda)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Cryo-em structure of e. coli rna polymerase backtracked elongation complex harboring a terminal mismatch (400 kDa, Complex)
- Rna (Complex from Escherichia phage Lambda)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Non-template dna (4 kDa, DNA from Escherichia phage Lambda)
Resolution: 7.3 Å
EM Method: Single-particle
Fitted PDBs: 8fiy
Q-score: 0.134
Wee LM, Tong AB, Florez Ariza AJ, Canari-Chumpitaz C, Grob P, Nogales E, Bustamante CJ
Cell (2023) 186 pp. 1244-1262.e34 [ Pubmed: 36931247 DOI: doi:10.1016/j.cell.2023.02.008 ]
- Non-template dna (4 kDa, DNA from Escherichia phage Lambda)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Rna (2 kDa, RNA from Escherichia phage Lambda)
- Zinc ion (65 Da, Ligand)
- Template dna (7 kDa, DNA from Escherichia phage Lambda)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Cryo-em structure of e. coli rna polymerase elongation complex (in the transcription-translation complex) harboring a terminal mismatch (400 kDa, Complex)