Cryo-EM structure of LPA1-Gi complex with LPA
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 9izf
Q-score: 0.487
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ Pubmed: 39725093 DOI: doi:10.1016/j.jsb.2024.108164 ]
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Multiprotein complex (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
Cryo-EM structure of LPA1-Gq complex with LPA
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9izg
Q-score: 0.557
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ Pubmed: 39725093 DOI: doi:10.1016/j.jsb.2024.108164 ]
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Multiprotein complex (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
Cryo-EM structure of LPA1-G13 complex with LPA
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9izh
Q-score: 0.553
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ DOI: doi:10.1016/j.jsb.2024.108164 Pubmed: 39725093 ]
- Multiprotein complex (Complex from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
Structure of murine heavy chain apoferritin collected at 200 keV with the Gatan K3 detector
Apoferritin data taken with laser-off
Remis J, Petrov PN, Zhang JT, Axelrod JJ, Cheng H, Sandhaus S, Mueller H, Glaeser RM
J Struct Biol (2024) 216 [ Pubmed: 39536845 DOI: 10.1016/j.jsb.2024.108150 ]
Image sets:
Apoferritin data taken with laser-on
Glaser RM, Remis JW, Remis J, Petrov PN, Zhang JT, Axelrod JJ, Cheng H, Sandhaus S, Mueller H, Glaeser RM
J Struct Biol (2024) 216 [ Pubmed: 39536845 DOI: 10.1016/j.jsb.2024.108150 ]
Image sets:
Rubisco structure determined using laser phase contrast TEM (laser-on)
Remis J, Petrov PN, Zhang JT, Axelrod JJ, Cheng H, Sandhaus S, Mueller H, Glaeser RM
J Struct Biol (2024) 216 pp. 108150-108150 [ DOI: doi:10.1016/j.jsb.2024.108150 Pubmed: 39536845 ]
- Rubisco (Complex from Halobacillus)