Stabilised BA.1 SARS-CoV-2 spike with H6 nanobodies in '3 up' RBD conformation
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8oyt
Q-score: 0.161
Cornish K, Huo J, Jones L, Sharma P, Thrush JW, Abdelkarim S, Kipar A, Ramadurai S, Weckener M, Mikolajek H, Liu S, Buckle I, Bentley E, Kirby A, Han X, Laidlaw SM, Hill M, Eyssen L, Norman C, Le Bas A, Clarke J, James W, Stewart JP, Carroll M, Naismith JH, Owens RJ
Open Biol (2024) 14 pp. 230252-230252 [ DOI: doi:10.1098/rsob.230252 Pubmed: 38835241 ]
- H6 nanobody (Complex from Lama glama)
- Stabilised hexapro omicron ba.1 spike trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- H6 nanobody (14 kDa, Protein from Lama glama)
- Spike glycoprotein,fibritin (140 kDa, Protein from Tequatrovirus T4)
- Complex of stabilised hexapro omicron ba.1 spike trimer with three h6 nanobodies (Complex)
Stabilised BA.1 SARS-CoV-2 spike with H6 nanobodies in '2 up 1 down' RBD conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8oyu
Q-score: 0.145
Cornish K, Huo J, Jones L, Sharma P, Thrush JW, Abdelkarim S, Kipar A, Ramadurai S, Weckener M, Mikolajek H, Liu S, Buckle I, Bentley E, Kirby A, Han X, Laidlaw SM, Hill M, Eyssen L, Norman C, Le Bas A, Clarke J, James W, Stewart JP, Carroll M, Naismith JH, Owens RJ
Open Biol (2024) 14 pp. 230252-230252 [ DOI: doi:10.1098/rsob.230252 Pubmed: 38835241 ]
- H6 nanobody (Complex from Lama glama)
- Stabilised hexapro omicron ba.1 spike trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of stabilised hexapro omicron ba.1 spike trimer with two h6 nanobodies (Complex)
- Spike glycoprotein,fibritin (139 kDa, Protein from Enterobacteria phage T4)
- H6 nanobody (13 kDa, Protein from Lama glama)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11 nanobody complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7z6v
Q-score: 0.434
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein,fibritin (139 kDa, Protein from Escherichia virus T4)
- Spike glycoprotein trimer with fibritin tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody h11 (Complex from Lama glama)
- Complex between spike and nanobody h11 (15 kDa, Complex)
- Nanobody h11 (14 kDa, Protein from Lama glama)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-H6 nanobody complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7z7x
Q-score: 0.461
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein trimer with fibritin tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody h11-h6 (14 kDa, Protein from Lama glama)
- Complex between spike and nanobody h11-h6 (15 kDa, Complex)
- Nanobody h11-h6 (Complex from Lama glama)
- Spike glycoprotein,fibritin (139 kDa, Protein from Escherichia virus T4)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-B5 nanobody complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7z85
Q-score: 0.447
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- Nanobody h11-b5 (Complex from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein trimer with fibritin tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Complex between spike and nanobody h11-b5 (15 kDa, Complex)
- Nanobody h11-b5 (14 kDa, Protein from Lama glama)
- Spike glycoprotein,fibritin (139 kDa, Protein from Escherichia virus T4)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-H4 Q98R H100E nanobody complex in 1Up2Down conformation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7z86
Q-score: 0.379
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- Nanobody h11-h4 q98r h100e (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein trimer with fibritin tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody h11-h4 q98r h100e (Complex from Lama glama)
- Complex between spike and nanobody h11-h4 q98r h100e (15 kDa, Complex)
- Spike glycoprotein,fibritin (139 kDa, Protein from Escherichia virus T4)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-A10 nanobody complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7z9q
Q-score: 0.346
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein trimer (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody h11-a10 (14 kDa, Protein from Lama glama)
- Spike glycoprotein,fibritin (139 kDa, Protein from Tequatrovirus T4)
- Nanobody h11-a10 (Complex from Lama glama)
- Complex between spike and nanobody h11-a10 (15 kDa, Complex)
CRYO-EM STRUCTURE OF SARS-COV-2 SPIKE : H11-H4 Q98R H100E nanobody complex in 2Up1Down conformation
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7z9r
Q-score: 0.319
Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH
PNAS (2022) 119 pp. e2205412119-e2205412119 [ DOI: doi:10.1073/pnas.2205412119 Pubmed: 35858383 ]
- Nanobody h11-h4 q98r h100e (14 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein trimer with fibritin tag (Complex from Severe acute respiratory syndrome coronavirus 2)
- Nanobody h11-h4 q98r h100e (Complex from Lama glama)
- Complex between spike and nanobody h11-h4 q98r h100e (15 kDa, Complex)
- Spike glycoprotein,fibritin (139 kDa, Protein from Escherichia virus T4)