Cryo-EM structure of T4 Bacteriophage Clamp Loader with Sliding Clamp and DNA
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8unf
Q-score: 0.447
Marcus K, Huang Y, Subramanian S, Gee CL, Gorday K, Ghaffari-Kashani S, Luo XR, Zheng L, O'Donnell M, Subramaniam S, Kuriyan J
Nat Struct Mol Biol (2024) 31 pp. 424-435 [ DOI: doi:10.1038/s41594-023-01177-3 Pubmed: 38177685 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Sliding clamp (24 kDa, Protein from Tequatrovirus T4)
- [[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium (492 Da, Ligand)
- Template dna (7 kDa, DNA from Tequatrovirus T4)
- T4 bacteriophage clamp loader subunit a (gp62) (Complex from Tequatrovirus T4)
- T4 bacteriophage clamp loader (gp44 + gp62) (Complex from Tequatrovirus T4)
- Sliding-clamp-loader large subunit (35 kDa, Protein from Tequatrovirus T4)
- T4 bacteriophage sliding clamp (gp45) (Complex from Tequatrovirus T4)
- T4 bacteriophage clamp loader subunits b,c,d,e(gp44) (Complex from Tequatrovirus T4)
- Magnesium ion (24 Da, Ligand)
- T4 bacteriophage clamp loader with sliding clamp and dna (gp44+gp62+gp45+dna) (Complex from Tequatrovirus T4)
- Sliding-clamp-loader small subunit (21 kDa, Protein from Tequatrovirus T4)
- Primed dna duplex (Complex from Tequatrovirus T4)
- Primer dna (6 kDa, DNA from Tequatrovirus T4)
Cryo-EM structure of T4 Bacteriophage Clamp Loader with Sliding Clamp
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8unh
Q-score: 0.445
Marcus K, Huang Y, Subramanian S, Gee CL, Gorday K, Ghaffari-Kashani S, Luo XR, Zheng L, O'Donnell M, Subramaniam S, Kuriyan J
Nat Struct Mol Biol (2024) 31 pp. 424-435 [ DOI: doi:10.1038/s41594-023-01177-3 Pubmed: 38177685 ]
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- T4 bacteriophage clamp loader (gp44 + gp62) (Complex from Tequatrovirus T4)
- Sliding-clamp-loader large subunit (35 kDa, Protein from Tequatrovirus T4)
- T4 bacteriophage sliding clamp (gp45) (Complex from Tequatrovirus T4)
- T4 bacteriophage clamp loader with sliding clamp (Complex from Tequatrovirus T4)
- Magnesium ion (24 Da, Ligand)
- Sliding-clamp-loader small subunit (21 kDa, Protein from Tequatrovirus T4)
- Sliding clamp (24 kDa, Protein from Tequatrovirus T4)
Negative-staining map of T4 DNA ligase-AMP Binary Complex III
To Be Published
To Be Published
To Be Published
Negative-staining map of T4 DNA ligase-AMP-DNA Ternary Complex II
To Be Published
Negative-staining map of T4 DNA ligase-AMP-DNA Ternary Complex III
To Be Published
Negative-staining map of T4 DNA ligase-AMP-DNA Ternary Complex IV
To Be Published
Negative-staining map of T4 DNA ligase-AMP Binary Complex I
To Be Published
- T4 dna ligase conformation open i (55 kDa, Complex from bacteriophage T)
Negative-staining map of T4 DNA ligase-AMP Binary Complex II
To Be Published
- T4 dna ligase conformation open ii (55 kDa, Complex from bacteriophage T)