Single particle reconstruction of the symmetric core an engineered protein scaffold
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6nht
Q-score: 0.568
Nat Commun (2019) 10 pp. 1864-1864 [ Pubmed: 31015551 DOI: doi:10.1038/s41467-019-09836-0 ]
- Subunit b (Complex from Pseudomonas aeruginosa PAO1)
- Darp14 - subunit a with darpin (34 kDa, Protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3))
- Subunit a with darpin subunit b (Complex)
- Subunit a with darpin (Complex from Pyrococcus horikoshii OT3)
- Darp14 - subunit b (14 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nhv
Q-score: 0.428
Nat Commun (2019) 10 pp. 1864-1864 [ Pubmed: 31015551 DOI: doi:10.1038/s41467-019-09836-0 ]
- Subunit b (Complex from Pseudomonas aeruginosa PAO1)
- Superfolder gfp (Complex from Aequorea victoria)
- Subunit a-darpin (34 kDa, Protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3))
- Superfolder gfp (26 kDa, Protein from Aequorea victoria)
- Subunit a with darpin (Complex from Pyrococcus horikoshii OT3)
- Subunit a with darpin + subunit b + superfolder gfp (Complex)
- Darp14 - subunit b (14 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
Liu Y, Gonen S, Gonen T, Yeates TO
PNAS (2018) 115 pp. 3362-3367 [ DOI: doi:10.1073/pnas.1718825115 Pubmed: 29507202 ]
- Darp14 (Complex from synthetic construct)
- Darp14 - darpin (Complex from synthetic construct)
- Darp14 - subunit a (Complex from Pyrococcus horikoshii OT3)
- Darp14 - subunit b (Complex from Pseudomonas aeruginosa PAO1)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 6c9i
Q-score: 0.57
Liu Y, Gonen S, Gonen T, Yeates TO
PNAS (2018) 115 pp. 3362-3367 [ DOI: doi:10.1073/pnas.1718825115 Pubmed: 29507202 ]
- Darp14 (Complex from synthetic construct)
- Darp14 - subunit b (Complex from Pseudomonas aeruginosa PAO1)
- Darp14 - subunit a (Complex from Pyrococcus horikoshii OT3)
- Darp14 - subunit a with darpin (18 kDa, Protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3))
- Darp14 - subunit b (14 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 6c9k
Q-score: 0.213
Liu Y, Gonen S, Gonen T, Yeates TO
PNAS (2018) 115 pp. 3362-3367 [ DOI: doi:10.1073/pnas.1718825115 Pubmed: 29507202 ]
- Darp14 (Complex from synthetic construct)
- Darp14 - subunit a with darpin (35 kDa, Protein from synthetic)
- Darp14 - subunit b (Complex from Pseudomonas aeruginosa PAO1)
- Darp14 - subunit a with darpin (Complex from synthetic construct)
- Darp14 - subunit b (14 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))