Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex with bound target dsDNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6b44
Q-score: 0.478
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell (2017) 171 pp. 414-426.e12 [ DOI: doi:10.1016/j.cell.2017.09.006 Pubmed: 28985564 ]
- Target dna strand (41-mer) (15 kDa, DNA from synthetic construct)
- Non-target dna strand (5'-d(p*cp*ap*gp*tp*cp*ap*tp*cp*ap*cp*cp*ap*a)-3') (8 kDa, DNA from synthetic construct)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Pseudomonas aeruginosa strain smc4485 crispr repeat sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Type i-f crispr crrna-guided csy surveillance complex with bound target dsdna (350 kDa, Complex from Pseudomonas aeruginosa)
Cryo-EM structure of Type I-F CRISPR crRNA-guided Csy surveillance complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6b45
Q-score: 0.413
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell (2017) 171 pp. 414-426.e12 [ DOI: doi:10.1016/j.cell.2017.09.006 Pubmed: 28985564 ]
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Pseudomonas aeruginosa strain smc4485 crispr repeat sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Type i-f crispr crrna-guided csy surveillance complex (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6b46
Q-score: 0.487
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell (2017) 171 pp. 414-426.e12 [ DOI: doi:10.1016/j.cell.2017.09.006 Pubmed: 28985564 ]
- Type i-f crispr crrna-guided csy surveillance complex with bound anti-crispr protein acrf1 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Pseudomonas aeruginosa strain smc4485 crispr repeat sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Anti-crispr protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6b48
Q-score: 0.41
Guo TW, Bartesaghi A, Yang H, Falconieri V, Rao P, Merk A, Eng ET, Raczkowski AM, Fox T, Earl LA, Patel DJ, Subramaniam S
Cell (2017) 171 pp. 414-426.e12 [ DOI: doi:10.1016/j.cell.2017.09.006 Pubmed: 28985564 ]
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Pseudomonas aeruginosa strain smc4485 crispr repeat sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Type i-f crispr crrna-guided csy surveillance complex with bound anti-crispr protein acrf10 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Anti-crispr protein acrf10 (11 kDa, Protein from Shewanella xiamenensis)