Cryo-EM structure of the 48-nm repeat unit of the doublet microtubule from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6u0h
Q-score: 0.357
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin lattice (Complex from Tetrahymena thermophila)
- 48nm-repeat of microtubule doublet (Cellular component from Tetrahymena thermophila)
Cryo-EM map of the A-tubule prepared by sonication
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
Cryo-EM map of the A-tubule prepared by 0.2% Sarkosyl treatment
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH
PNAS (2019) 116 pp. 19930-19938 [ DOI: doi:10.1073/pnas.1911119116 Pubmed: 31527277 ]
Cryo-EM structure of 8nm repeat tubulin lattice of the ciliary microtubule doublet
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 5ubq
Q-score: 0.144
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- 8nm-repeat tubulin lattice of microtubule doublet (Cellular component from Tetrahymena thermophila)
- Tubulin beta chain (48 kDa, Protein from Tetrahymena thermophila)
Cryo-EM structure of 16nm repeat ciliary microtubule doublet
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
Cryo-EM structure of 48nm repeat ciliary microtubule doublet
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
Cryo-EM map of protofilament of microtubule doublet
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5ucy
Q-score: 0.264
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S, Bui KH
Nat Commun (2017) 8 pp. 15035-15035 [ DOI: doi:10.1038/ncomms15035 Pubmed: 28462916 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Tetrahymena thermophila)
- Protofilament from ciliary microtubule doublet (Cellular component from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (48 kDa, Protein from Tetrahymena thermophila)