In situ cryo-electron tomogram of a multi-lamellar vesicle in a NPC2-/- HeLa cell. #1
Kraus F, He Y, Swarup S, Overmyer KA, Jiang Y, Brenner J, Capitanio C, Bieber A, Jen A, Nightingale NM, Anderson BJ, Lee C, Paulo JA, Smith IR, Plitzko JM, Gygi SP, Schulman BA, Wilfling F, Coon JJ, Harper JW
Sci Adv (2025) 11 pp. eadu5787-eadu5787 [ DOI: doi:10.1126/sciadv.adu5787 Pubmed: 39841834 ]
In situ cryo-electron tomogram of a multi-lamellar vesicle in a NPC2-/- HeLa cell. #2
Kraus F, He Y, Swarup S, Overmyer KA, Jiang Y, Brenner J, Capitanio C, Bieber A, Jen A, Nightingale NM, Anderson BJ, Lee C, Paulo JA, Smith IR, Plitzko JM, Gygi SP, Schulman BA, Wilfling F, Coon JJ, Harper JW
Sci Adv (2025) 11 pp. eadu5787-eadu5787 [ DOI: doi:10.1126/sciadv.adu5787 Pubmed: 39841834 ]
Cryo-EM structure of a trapped ARIH1-diUB-CRL2-KLHDC10 complex - consensus map
Scott DC, Chittori S, Purser N, King MT, Maiwald SA, Churion K, Nourse A, Lee C, Paulo JA, Miller DJ, Elledge SJ, Harper JW, Kleiger G, Schulman BA
Nat Commun (2024) 15 pp. 9899-9899 [ DOI: doi:10.1038/s41467-024-54126-z Pubmed: 39548056 ]
- Klhdc10 (Protein from Homo sapiens)
- Elob (Protein from Homo sapiens)
- Cul2 (Protein from Homo sapiens)
- Nedd8 (Protein from Homo sapiens)
- Ub (Protein from Homo sapiens)
- Trapped arih1-diub-crl2-klhdc10 complex (270 kDa, Complex)
- Arih1 (Protein from Homo sapiens)
- Eloc (Protein from Homo sapiens)
- Rbx1 (Protein from Homo sapiens)
Focused map of Cryo-EM structure of Ubiquitin C-degron bound to KLHDC10-EloB/C
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9d8p
Q-score: 0.522
Scott DC, Chittori S, Purser N, King MT, Maiwald SA, Churion K, Nourse A, Lee C, Paulo JA, Miller DJ, Elledge SJ, Harper JW, Kleiger G, Schulman BA
Nat Commun (2024) 15 pp. 9899-9899 [ DOI: doi:10.1038/s41467-024-54126-z Pubmed: 39548056 ]
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Ubiquitin-c (8 kDa, Protein from Homo sapiens)
- Trapped arih1-diub-crl2-klhdc10 complex (Complex)
- Kelch domain-containing protein 10 (45 kDa, Protein from Homo sapiens)
- Elongin-b (13 kDa, Protein from Homo sapiens)
Structure of CUL9-RBX1 ubiquitin E3 ligase complex - hexameric assembly
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8q7e
Q-score: 0.301
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Structure of cul9-rbx1 ubiquitin e3 ligase complex - hexameric assembly (Complex from Homo sapiens)
- Cullin-9 (281 kDa, Protein from Homo sapiens)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8q7h
Q-score: 0.39
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]
- Zinc ion (65 Da, Ligand)
- Cullin-9 (281 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Structure of cul9-rbx1 ubiquitin e3 ligase complex in unneddylated and neddylated conformation - focused cullin dimer (Complex from Homo sapiens)
- Nedd8 (9 kDa, Protein from Homo sapiens)
- Unknown (12 kDa, Protein from Homo sapiens)
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 CPH domain
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]
Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 DOC domain
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) 31 pp. 1083-1094 [ Pubmed: 38605244 DOI: doi:10.1038/s41594-024-01257-y ]