CryoEM Structure of HCA2-Gi1 in complex with MK-1903
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8utd
Q-score: 0.347
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ Pubmed: 39631393 DOI: doi:10.1016/j.cell.2024.11.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Human hca2-gai1 in complex with mk1903 (Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- (4ar,5ar)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole-3-carboxylic acid (164 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Hydroxycarboxylic acid receptor 2 (42 kDa, Protein from Homo sapiens)
CryoEM Structure of HCA2 DREADD Gi1 in complex with FCH-2296413
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8uuj
Q-score: 0.53
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ Pubmed: 39631393 DOI: doi:10.1016/j.cell.2024.11.001 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Mouse hcad-gai1 in complex with fch-2296413 (Complex from Homo sapiens)
- (3m,4ar,5ar)-3-(1h-tetrazol-5-yl)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole (188 Da, Ligand)
- Hydroxycarboxylic acid receptor 2 (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
COVA2-15 fragment antigen binding in complex with SARS-CoV-2 6P-mut7 S protein
de Taeye SW, Faye L, Morel B, Schriek AI, Umotoy JC, Yuan M, Kuzmina NA, Turner HL, Zhu X, Grunwald-Gruber C, Poniman M, Burger JA, Caniels TG, Fitchette AC, Desgagnes R, Stordeur V, Mirande L, Beauverger G, de Bree G, Ozorowski G, Ward AB, Wilson IA, Bukreyev A, Sanders RW, Vezina LP, Beaumont T, van Gils MJ, Gomord V
Plant Biotechnol J (2025) 23 pp. 4-16 [ Pubmed: 39563066 DOI: doi:10.1111/pbi.14458 ]
- Cova2-15 kappa chain variable region (12 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cova2-15 heavy chain variable region (13 kDa, Protein from Homo sapiens)
- Cova2-15 fragment antigen binding in complex with sars-cov-2 6p-mut7 s protein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (141 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Overall map of ncPRC1RYBP bound to doubly modified H2AK119Ub nucleosome
Lopez VG, Valencia-Sanchez MI, Abini-Agbomson S, Thomas JF, Lee R, De Ioannes P, Sosa BA, Armache JP, Armache KJ
Nature (2024) 636 pp. 755-761 [ DOI: doi:10.1038/s41586-024-08183-5 Pubmed: 39537923 ]
- Histones octamer (Complex from Xenopus laevis)
- Histone h4 (Complex from Xenopus laevis)
- Histone h2a (Complex from Xenopus laevis)
- Ring1 and yy1-binding protein (Complex from Homo sapiens)
- Dna (187-mer) (DNA from synthetic construct)
- Histone h3.2 (Protein from Xenopus laevis)
- Histone h2b 1.1 (Complex from Xenopus laevis)
- E3 ubiquitin-protein ligase ring2, ubiquitin-conjugating enzyme e2 d3 (Protein from Homo sapiens)
- E3 ubiquitin-protein ligase ring2, ubiquitin-conjugating enzyme e2 d3 (Complex from Homo sapiens)
- Dna (187-mer) (Complex from synthetic construct)
- Histone h2b 1.1 (Protein from Xenopus laevis)
- Ncprc1-rybp (Complex from Homo sapiens)
- Ubiquitin (Protein from Homo sapiens)
- Overall map of ncprc1rybp bound to doubly modified h2ak119ub nucleosome (Complex from Homo sapiens)
- Histone h2a (Protein from Xenopus laevis)
- Ring1 and yy1-binding protein (Protein from Homo sapiens)
- Polycomb complex protein bmi-1 (Protein from Homo sapiens)
- Histone h4 (Protein from Xenopus laevis)
- Ubiquitin (Complex from Homo sapiens)
- Histone h3.2 (Complex from Xenopus laevis)
- Polycomb complex protein bmi-1 (Complex from Homo sapiens)
ncPRC1RYBP Delta-linker mutant bound to singly modified H2AK119Ub nucleosome
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 9dg3
Q-score: 0.382
Lopez VG, Valencia-Sanchez MI, Abini-Agbomson S, Thomas JF, Lee R, De Ioannes P, Sosa BA, Armache JP, Armache KJ
Nature (2024) 636 pp. 755-761 [ DOI: doi:10.1038/s41586-024-08183-5 Pubmed: 39537923 ]
- Zinc ion (65 Da, Ligand)
- Ncprc1rybp delta-linker mutant bound to singly modified h2ak119ub nucleosome (Complex from Homo sapiens)
- Dna (187-mer) (57 kDa, DNA from synthetic construct)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Ring1 and yy1-binding protein (24 kDa, Protein from Homo sapiens)
- Dna (187-mer) (57 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
ncPRC1RYBP bound to unmodified nucleosome
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 9dgg
Q-score: 0.47
Lopez VG, Valencia-Sanchez MI, Abini-Agbomson S, Thomas JF, Lee R, De Ioannes P, Sosa BA, Armache JP, Armache KJ
Nature (2024) 636 pp. 755-761 [ DOI: doi:10.1038/s41586-024-08183-5 Pubmed: 39537923 ]
- Zinc ion (65 Da, Ligand)
- Dna (187-mer) (57 kDa, DNA from synthetic construct)
- Ncprc1rybp bound to unmodified nucleosome (Complex from Homo sapiens)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Polycomb complex protein bmi-1 (37 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase ring2 (37 kDa, Protein from Homo sapiens)
- Dna (187-mer) (57 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
C-terminal LRRK2 bound to E11 DARPin
Mathea S, Dederer V, Sanz-Murillo M, Leschziner A, Knapp S
To Be Published
- E11 darpin (19 kDa, Protein from synthetic construct)
- C-terminal lrrk2 bound to e11 darpin (156 kDa, Complex from Homo sapiens)
- Leucine-rich repeat serine/threonine-protein kinase 2 (136 kDa, Protein from Homo sapiens)
Additional cryo-EM structure of cardiac amyloid AL59 - bent polymorph
Schulte T, Chaves-Sanjuan A, Speranzini V, Sicking K, Milazzo M, Mazzini G, Rognoni P, Caminito S, Milani P, Marabelli C, Corbelli A, Diomede L, Fiordaliso F, Anastasia L, Pappone C, Merlini G, Bolognesi M, Nuvolone M, Fernandez-Busnadiego R, Palladini G, Ricagno S
To Be Published
Additional cryo-EM structure of cardiac amyloid AL59 - mixed polymorph
Schulte T, Chaves-Sanjuan A, Speranzini V, Sicking K, Milazzo M, Mazzini G, Rognoni P, Caminito S, Milani P, Marabelli C, Corbelli A, Diomede L, Fiordaliso F, Anastasia L, Pappone C, Merlini G, Bolognesi M, Nuvolone M, Fernandez-Busnadiego R, Palladini G, Ricagno S
Nat Commun (2024) 15 pp. 6359-6359 [ Pubmed: 39069558 DOI: doi:10.1038/s41467-024-50686-2 ]
Maps of Collagen VI half- and full-beads
Schulte T, Chaves-Sanjuan A, Speranzini V, Sicking K, Milazzo M, Mazzini G, Rognoni P, Caminito S, Milani P, Marabelli C, Corbelli A, Diomede L, Fiordaliso F, Anastasia L, Pappone C, Merlini G, Bolognesi M, Nuvolone M, Fernandez-Busnadiego R, Palladini G, Ricagno S
Nat Commun (2024) 15 pp. 6359-6359 [ Pubmed: 39069558 DOI: doi:10.1038/s41467-024-50686-2 ]