Resolution: 3.55 Å
EM Method: Single-particle
Fitted PDBs: 9j7i
Q-score: 0.482
Yamaguchi H, Kitajima S, Suzuki H, Suzuki S, Nishikawa K, Kamegawa A, Fujiyoshi Y, Takahashi K, Tagami U, Maruyama Y, Kuroda M, Sugiki M
Sci Rep (2025) 15 pp. 3894-3894 [ Pubmed: 39890873 DOI: doi:10.1038/s41598-025-87999-1 ]
- Gamma-glutamyl-valyl-glycine (303 Da, Protein from synthetic construct)
- Extracellular calcium-sensing receptor (101 kDa, Protein from Homo sapiens)
- Extracellular regions of calcium-sensing receptor (Complex from Homo sapiens)
Cryo-EM structure of LPA1-Gi complex with LPA
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 9izf
Q-score: 0.487
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ Pubmed: 39725093 DOI: doi:10.1016/j.jsb.2024.108164 ]
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Multiprotein complex (Complex from Homo sapiens)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
Cryo-EM structure of LPA1-Gq complex with LPA
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9izg
Q-score: 0.557
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ Pubmed: 39725093 DOI: doi:10.1016/j.jsb.2024.108164 ]
- Scfv16 (26 kDa, Protein from synthetic construct)
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Multiprotein complex (Complex from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
Cryo-EM structure of LPA1-G13 complex with LPA
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9izh
Q-score: 0.553
Suzuki S, Tanaka K, Kamegawa A, Nishikawa K, Suzuki H, Oshima A, Fujiyoshi Y
J Struct Biol (2024) 217 pp. 108164-108164 [ Pubmed: 39725093 DOI: doi:10.1016/j.jsb.2024.108164 ]
- Scfv16 (26 kDa, Protein from synthetic construct)
- Soluble cytochrome b562,lysophosphatidic acid receptor 1,lgbit tag (73 kDa, Protein from synthetic construct)
- (2r)-2-hydroxy-3-(phosphonooxy)propyl (9e)-octadec-9-enoate (436 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Multiprotein complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Mus musculus)
Cryo-EM structure of human testis-specific Na+,K+-ATPase alpha4 in ouabain-bound form
Resolution: 2.37 Å
EM Method: Single-particle
Fitted PDBs: 8zyj
Q-score: 0.639
Abe K, McDermott J, Valia Madapally H, Marimuthu P, Gopalasingam CC, Gerle C, Shigematsu H, Khandelia H, Blanco G
Int J Mol Sci (2024) 25 [ DOI: doi:10.3390/ijms252212397 Pubmed: 39596464 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium/potassium-transporting atpase subunit alpha-4 (108 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Magnesium ion (24 Da, Ligand)
- Sodium/potassium-transporting atpase subunit beta-1 (35 kDa, Protein from Homo sapiens)
- Ouabain (584 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Alpha4-beta1 heterodimer (135 MDa, Complex from Homo sapiens)
Wild-type native PMEL amyloid - polymorph 1
Resolution: 1.79 Å
EM Method: Single-particle
Fitted PDBs: 9jst
Q-score: 0.777
To Be Published
Wild-type native PMEL amyloid - polymorph 2
Resolution: 1.79 Å
EM Method: Single-particle
Fitted PDBs: 9jsu
Q-score: 0.758
To Be Published
G175S mutant native PMEL amyloid
Resolution: 1.79 Å
EM Method: Single-particle
Fitted PDBs: 9jsv
Q-score: 0.79
To Be Published
Wild-type PMEL CAF amyloid -in vitro polymerized
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 9jsw
Q-score: 0.735
To Be Published
- Wild-type pmel caf amyloid expressed in e. coli (Cell from Homo sapiens)
- M-alpha (3 kDa, Protein from Homo sapiens)
G175S PMEL CAF amyloid - in vitro polymerized
Resolution: 1.79 Å
EM Method: Single-particle
Fitted PDBs: 9jsx
Q-score: 0.778
To Be Published
- M-alpha (3 kDa, Protein from Homo sapiens)
- G175s mutant pmel caf domain, expressed in e. coli (Cell from Homo sapiens)