Cryo-EM structure of the N2-4N2C402 complex at a resolution of 1.9 angstrom
Resolution: 1.9 Å
EM Method: Single-particle
Fitted PDBs: 8zr4
Q-score: 0.793
Wang X, Yang Q, Chu BX, Wu XD, Zhang ZL
Cell Host Microbe (2024) [ ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 4n2c402_fab_l (12 kDa, Protein from Homo sapiens)
- A complex of n2 with 4n2c402-fab (500 kDa, Complex from Homo sapiens)
- Neuraminidase (43 kDa, Protein from Influenza A virus)
- Water (18 Da, Ligand)
- 4n2c402_fab_h (15 kDa, Protein from Homo sapiens)
Resolution: 1.99 Å
EM Method: Helical reconstruction
Fitted PDBs: 8bg0
Q-score: 0.743
Yang Y, Zhang W, Murzin AG, Schweighauser M, Huang M, Lovestam S, Peak-Chew SY, Saito T, Saido TC, Macdonald J, Lavenir I, Ghetti B, Graff C, Kumar A, Nordberg A, Goedert M, Scheres SHW
Acta Neuropathol (2023) 145 pp. 325-333 [ DOI: doi:10.1007/s00401-022-02533-1 Pubmed: 36611124 ]
High resolution of apo-ferritin embedded in crystalline ice
Structure (2023) 31 pp. 213-220.e3 [ Pubmed: 36586403 DOI: doi:10.1016/j.str.2022.12.001 ]
- Human apo-ferritin (440 kDa, Complex from Homo sapiens)
Resolution: 1.89 Å
EM Method: Single-particle
Fitted PDBs: 7v66
Q-score: 0.018
Wu C, Shi H, Zhu D, Fan K, Zhang X
QRB Discov (2021) 2 [ DOI: doi:10.1017/qrd.2021.8 Pubmed: 37529673 ]
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Human apoferritin (440 kDa, Complex from Homo sapiens)
hBest2 1uM Ca2+ (Ca2+-bound) closed state
Resolution: 1.78 Å
EM Method: Single-particle
Fitted PDBs: 8d1e
Q-score: 0.789
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Hbest2 1um ca2+ (ca2+-bound) closed state (234 kDa, Complex from Homo sapiens)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
hBest2 5mM Ca2+ (Ca2+-bound) closed state
Resolution: 1.82 Å
EM Method: Single-particle
Fitted PDBs: 8d1f
Q-score: 0.787
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Hbest2 5mm ca2+ (ca2+-bound) closed state (234 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
hBest2 Ca2+-unbound closed state
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 8d1h
Q-score: 0.78
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Hbest2 ca2+-unbound closed state (234 kDa, Complex from Homo sapiens)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
hBest1 1uM Ca2+ (Ca2+-bound) closed state
Resolution: 1.82 Å
EM Method: Single-particle
Fitted PDBs: 8d1i
Q-score: 0.786
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Calcium ion (40 Da, Ligand)
- Bestrophin-1 (67 kDa, Protein from Homo sapiens)
- Hbest1 1um ca2+ (ca2+-bound) closed state (338 kDa, Complex from Homo sapiens)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 1.58 Å
EM Method: Single-particle
Fitted PDBs: 7r5o
Q-score: 0.869
Sheng Y, Harrison PJ, Vogirala V, Yang Z, Strain-Damerell C, Frosio T, Himes BA, Siebert CA, Zhang P, Clare DK
Faraday Disc.Chem.Soc (2022) 240 pp. 261-276 [ Pubmed: 35938521 DOI: doi:10.1039/d2fd00049k ]
- Ferritin heavy chain, n-terminally processed (20 kDa, Protein from Homo sapiens)
- Human apoferritin (500 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
1.96 A structure of human apoferritin obtained from Talos Arctica microscope
Resolution: 1.96 Å
EM Method: Single-particle
Fitted PDBs: 7vd8
Q-score: 0.822
Fan H, Wang B, Zhang Y, Zhu Y, Song B, Xu H, Zhai Y, Qiao M, Sun F
Nat Commun (2021) 12 pp. 7257-7257 [ DOI: doi:10.1038/s41467-021-27596-8 Pubmed: 34907237 ]
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Human apoferritin (480 kDa, Complex from Homo sapiens)
- Ferritin heavy chain (20 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)