KCTD5/Cullin3/Gbeta1gamma2 Complex State B: Composite Map from RELION Multi-body Refinement
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 8u82
Q-score: 0.254
Nguyen DM, Rath DH, Devost D, Petrin D, Rizk R, Ji AX, Narayanan N, Yong D, Zhai A, Kuntz DA, Mian MUQ, Pomroy NC, Keszei AFA, Benlekbir S, Mazhab-Jafari MT, Rubinstein JL, Hebert TE, Prive GG
PNAS (2024) 121 pp. e2315018121-e2315018121 [ Pubmed: 38625940 DOI: doi:10.1073/pnas.2315018121 ]
- Gbg2_human (Protein from Homo sapiens)
- Kctd5_human (Protein from Homo sapiens)
- Cullin3 1-381 (Protein from Homo sapiens)
- Complex of substrate adaptor kctd5 with cullin-3 and substrate gbeta-gamma (Complex from Homo sapiens)
- Gbb1_human (Protein from Homo sapiens)
Structure of VSD4-NaV1.7-NaVPas channel chimera bound to the hybrid inhibitor GNE-1305
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8f0p
Q-score: 0.776
Kschonsak M, Jao CC, Arthur CP, Rohou AL, Bergeron P, Ortwine DF, McKerrall SJ, Hackos DH, Deng L, Chen J, Li T, Dragovich PS, Volgraf M, Wright MR, Payandeh J, Ciferri C, Tellis JC
eLife (2023) 12 [ Pubmed: 36975198 DOI: doi:10.7554/eLife.84151 ]
- Sodium channel protein pafpc1,sodium channel protein type 9 subunit alpha chimera (184 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- N-[6-(cyclopentylmethoxy)-1,3-benzothiazol-2-yl]-4-{[(1s,2s)-2-(dimethylamino)cyclohexyl]amino}-2-fluorobenzene-1-sulfonamide (546 Da, Ligand)
- Vsd4-nav1.7-navpas channel chimera bound to the hybrid inhibitor gne-1305 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)
Structure of VSD4-NaV1.7-NaVPas channel chimera bound to the acylsulfonamide inhibitor GDC-0310
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 8f0q
Q-score: 0.739
Kschonsak M, Jao CC, Arthur CP, Rohou AL, Bergeron P, Ortwine DF, McKerrall SJ, Hackos DH, Deng L, Chen J, Li T, Dragovich PS, Volgraf M, Wright MR, Payandeh J, Ciferri C, Tellis JC
eLife (2023) 12 [ Pubmed: 36975198 DOI: doi:10.7554/eLife.84151 ]
- Beta-d-mannopyranose (180 Da, Ligand)
- Structure of vsd4-nav1.7-navpas channel chimera bound to the acylsulfonamide inhibitor gdc-0310 (Complex from Homo sapiens)
- Sodium channel protein pafpc1,sodium channel protein type 9 subunit alpha chimera (184 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)
- 5-cyclopropyl-4-({1-[(1s)-1-(3,5-dichlorophenyl)ethyl]piperidin-4-yl}methoxy)-2-fluoro-n-(methanesulfonyl)benzamide (543 Da, Ligand)
Structure of VSD4-NaV1.7-NaVPas channel chimera bound to the arylsulfonamide inhibitor GNE-3565
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8f0r
Q-score: 0.556
Kschonsak M, Jao CC, Arthur CP, Rohou AL, Bergeron P, Ortwine DF, McKerrall SJ, Hackos DH, Deng L, Chen J, Li T, Dragovich PS, Volgraf M, Wright MR, Payandeh J, Ciferri C, Tellis JC
eLife (2023) 12 [ Pubmed: 36975198 DOI: doi:10.7554/eLife.84151 ]
- Sodium channel protein pafpc1,sodium channel protein type 9 subunit alpha chimera (184 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Vsd4-nav1.7-navpas channel chimera bound to the arylsulfonamide inhibitor gne-3565 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 5-chloro-4-({(1s,2s,4s)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl}amino)-2-fluoro-n-(pyrimidin-4-yl)benzene-1-sulfonamide (572 Da, Ligand)
Structure of VSD4-NaV1.7-NaVPas channel chimera bound to the hybrid inhibitor GNE-9296
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8f0s
Q-score: 0.614
Kschonsak M, Jao CC, Arthur CP, Rohou AL, Bergeron P, Ortwine DF, McKerrall SJ, Hackos DH, Deng L, Chen J, Li T, Dragovich PS, Volgraf M, Wright MR, Payandeh J, Ciferri C, Tellis JC
eLife (2023) 12 [ Pubmed: 36975198 DOI: doi:10.7554/eLife.84151 ]
- Sodium channel protein pafpc1,sodium channel protein type 9 subunit alpha chimera (184 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Beta-diguetoxin-dc1a (8 kDa, Protein from Diguetia canities)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 5-chloro-4-(cyclopentylmethoxy)-n-(4-{[(1s,2s)-2-(dimethylamino)cyclohexyl]amino}-2-fluorobenzene-1-sulfonyl)-2-fluorobenzamide (570 Da, Ligand)
- Vsd4-nav1.7-navpas channel chimera bound to the hybrid inhibitor gne-9296 (Complex from Homo sapiens)
P5C3 is a potent fab neutralizer
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7p40
Q-score: 0.308
Fenwick C, Turelli P, Perez L, Pellaton C, Esteves-Leuenberger L, Farina A, Campos J, Lana E, Fiscalini F, Raclot C, Pojer F, Lau K, Demurtas D, Descatoire M, Joo VS, Foglierini M, Noto A, Abdelnabi R, Foo CS, Vangeel L, Neyts J, Du W, Bosch BJ, Veldman G, Leyssen P, Thiel V, LeGrand R, Levy Y, Trono D, Pantaleo G
Cell Rep (2021) 37 pp. 109814-109814 [ DOI: doi:10.1016/j.celrep.2021.109814 Pubmed: 34599871 ]
- Variable light chain p5c3 (vl) (23 kDa, Protein from Homo sapiens)
- Fab (Complex from Homo sapiens)
- Severe acute respiratory syndrome coronavirus 2 (Complex)
- Variable heavy chain p5c3 (vh) (23 kDa, Protein from Homo sapiens)
- Spike glycoprotein (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (141 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
the local resolution of Fab p5c3.
Fenwick C, Turelli P, Perez L, Pellaton C, Esteves-Leuenberger L, Farina A, Campos J, Lana E, Fiscalini F, Raclot C, Pojer F, Lau K, Demurtas D, Descatoire M, Joo VS, Foglierini M, Noto A, Abdelnabi R, Foo CS, Vangeel L, Neyts J, Du W, Bosch BJ, Veldman G, Leyssen P, Thiel V, LeGrand R, Levy Y, Trono D, Pantaleo G
Cell Rep (2021) 37 pp. 109814-109814 [ DOI: doi:10.1016/j.celrep.2021.109814 Pubmed: 34599871 ]
Structure of human TRPA1 in complex with agonist GNE551
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6x2j
Q-score: 0.509
Liu C, Reese R, Vu S, Rouge L, Shields SD, Kakiuchi-Kiyota S, Chen H, Johnson K, Shi YP, Chernov-Rogan T, Greiner DMZ, Kohli PB, Hackos D, Brillantes B, Tam C, Li T, Wang J, Safina B, Magnuson S, Volgraf M, Payandeh J, Zheng J, Rohou A, Chen J
Neuron (2021) 109 pp. 273-284.e4 [ DOI: doi:10.1016/j.neuron.2020.10.014 Pubmed: 33152265 ]
MicroED structure of a FIB-milled CypA Crystal
Resolution: 2.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 6u5g
Q-score: 0.702
Wolff AM, Young ID, Sierra RG, Brewster AS, Martynowycz MW, Nango E, Sugahara M, Nakane T, Ito K, Aquila A, Bhowmick A, Biel JT, Carbajo S, Cohen AE, Cortez S, Gonzalez A, Hino T, Im D, Koralek JD, Kubo M, Lazarou TS, Nomura T, Owada S, Samelson A, Tanaka T, Tanaka R, Thompson EM, van den Bedem H, Woldeyes RA, Yumoto F, Zhao W, Tono K, Boutet S, Iwata S, Gonen T, Sauter NK, Fraser JS, Thompson MC
IUCrJ (2020) 7 pp. 306-323 [ DOI: doi:10.1107/S205225252000072X ]
- Peptidyl-prolyl cis-trans isomerase a (18 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase a (Complex from Homo sapiens)
3.6 Angstrom resolution MAVS filament generated from helical reconstruction, truncated map
Resolution: 3.64 Å
EM Method: Helical reconstruction
Fitted PDBs: 3j6j
Q-score: 0.354
Wu B, Peisley A, Tetrault D, Li Z, Egelman EH, Magor KE, Walz T, Penczek PA, Hur S
Mol Cell (2014) 55 pp. 511-523 [ DOI: doi:10.1016/j.molcel.2014.06.010 Pubmed: 25018021 ]