Type III-B Cmr-beta Cryo-EM structure of the Apo state
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6s6b
Q-score: 0.456
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (51-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6s8b
Q-score: 0.48
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (46-mer) (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the type III-B Cmr-beta complex bound to non-cognate target RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6s8e
Q-score: 0.397
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Non-cognate target rna (15 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta complex bound to non-cognate target rna (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmr7 (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 6s91
Q-score: 0.463
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated proteins cmr5, cmr3, cmr1, cmr2, cmr7 (Complex from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (Complex from synthetic construct)
- Crrna (48-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp proteins cmr4 and cmr6, and crrna (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6sh8
Q-score: 0.437
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (Complex from synthetic construct)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 6shb
Q-score: 0.442
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Crrna (49-mer) (Complex from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (Complex from synthetic construct)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (49-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (14 kDa, RNA from synthetic construct)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 6sic
Q-score: 0.343
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex)
Reconstruction of the CRISPR-associated hexameric RNase Csx1 from Sulfolobus islandicus
Molina R, Stella S, Feng M, Sofos N, Jauniskis V, Pozdnyakova I, Lopez-Mendez B, She Q, Montoya G
Nat Commun (2019) 10 pp. 4302-4302 [ Pubmed: 31541109 DOI: doi:10.1038/s41467-019-12244-z ]
- Csx1 hexamer (320 kDa, Complex from Sulfolobus islandicus REY15A)