Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Upright conformation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6wxe
Q-score: 0.472
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- Rotavirus vp4, vp6, vp7 assembly in the spike conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxf
Q-score: 0.306
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Rotavirus vp4, vp6, vp7 assembly in an intermediate confromattion (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
Cryo-EM reconstruction of VP5*/VP8* assembly from rhesus rotavirus particles - Reversed conformation
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6wxg
Q-score: 0.509
Herrmann T, Torres R, Salgado EN, Berciu C, Stoddard D, Nicastro D, Jenni S, Harrison SC
Nature (2021) 590 pp. 666-670 [ Pubmed: 33442061 DOI: doi:10.1038/s41586-020-03124-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Rotavirus vp4, vp6, vp7 assembly in the foldback conformation (1 MDa, Complex from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid glycoprotein vp7 (37 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Intermediate capsid protein vp6 (44 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))
- Outer capsid protein vp4 (86 kDa, Protein from Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]))