E1435Q Ycf1 mutant in inward-facing narrow conformation
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 7m68
Q-score: 0.331
Khandelwal NK, Millan CR, Zangari SI, Avila S, Williams D, Thaker TM, Tomasiak TM
Nat Commun (2022) 13 pp. 1278-1278 [ DOI: doi:10.1038/s41467-022-28811-w Pubmed: 35277487 ]
- Ycf1 monomer (176 kDa, Complex from Saccharomyces cerevisiae S288C)
- Metal resistance protein ycf1 (176 kDa, Protein from Saccharomyces cerevisiae S288C)
E1435Q Ycf1 mutant in inward-facing wide conformation
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 7m69
Q-score: 0.318
Khandelwal NK, Millan CR, Zangari SI, Avila S, Williams D, Thaker TM, Tomasiak TM
Nat Commun (2022) 13 pp. 1278-1278 [ DOI: doi:10.1038/s41467-022-28811-w Pubmed: 35277487 ]
- Metal resistance protein ycf1 (176 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ycf1 (176 kDa, Complex from Saccharomyces cerevisiae S288C)
Engineered disulfide cross-linked closed conformation of the Yeast gamma-TuRC(SS)
Resolution: 3.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2w
Q-score: 0.435
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Engineered disulfide cross-linked closed conformation of the yeast gamma-turc(ss) (Complex from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Open conformation of the Yeast wild-type gamma-TuRC
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2x
Q-score: 0.295
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Open conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Closed conformation of the Yeast wild-type gamma-TuRC
Resolution: 4.03 Å
EM Method: Helical reconstruction
Fitted PDBs: 7m2y
Q-score: 0.249
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Spindle pole body component 110 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Closed conformation of the yeast wild-type gamma-turc (Complex from Saccharomyces cerevisiae)
Monomeric single-particle reconstruction of the Yeast gamma-TuSC
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7m2z
Q-score: 0.314
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.65168 Pubmed: 33949948 ]
- Spindle pole body component spc98 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Monomer of the yeast gamma-tusc (300 kDa, Complex from Saccharomyces cerevisiae)
- Spindle pole body component spc97 (96 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Lee JH, Bollschweiler D, Schafer T, Huber R
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abd4413 Pubmed: 33523989 ]
- Histone (Complex from Xenopus laevis)
- Histone deacetylase (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hdac-pc-nuc (Complex)
- Dna (Complex from unidentified plasmid)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6z6o
Q-score: 0.313
Lee JH, Bollschweiler D, Schafer T, Huber R
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abd4413 Pubmed: 33523989 ]
- Zinc ion (65 Da, Ligand)
- Histone deacetylase hda1 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone deacetylase hda1 (76 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hda1 complex subunit 3,hda1 complex subunit 3 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hdac-tc (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hda1 complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Resolution: 4.43 Å
EM Method: Single-particle
Fitted PDBs: 6z6p
Q-score: 0.189
Lee JH, Bollschweiler D, Schafer T, Huber R
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abd4413 Pubmed: 33523989 ]
- Histone h2b (10 kDa, Protein from Xenopus laevis)
- Histone deacetylase hda1 (76 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (8 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (11 kDa, Protein from Xenopus laevis)
- Histone h4 (9 kDa, Protein from Xenopus laevis)
- Hdac-pc-nuc (Complex)
- Dna (145-mer) (44 kDa, DNA from unidentified plasmid)
- Hda1 complex subunit 3,hda1 complex subunit 3 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone (Complex from Xenopus laevis)
- Histone h2a (11 kDa, Protein from Xenopus laevis)
- Histone deacetylase (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hda1 complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3 (11 kDa, Protein from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Histone deacetylase hda1 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3.2 (11 kDa, Protein from Xenopus laevis)
- Dna (145-mer) (44 kDa, DNA from unidentified plasmid)
- Dna (Complex from unidentified plasmid)
- Histone h2b (10 kDa, Protein from Xenopus laevis)
Lee JH, Bollschweiler D, Schafer T, Huber R
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abd4413 Pubmed: 33523989 ]
- Histone (Complex from Xenopus laevis)
- Histone deacetylase (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hdac-pc-nuc (Complex)
- Dna (Complex from unidentified plasmid)