Localized reconstruction of EEEV
Chen CL, Klose T, Sun C, Kim AS, Buda G, Rossmann MG, Diamond MS, Klimstra WB, Kuhn RJ
PNAS (2022) 119 pp. e2114119119-e2114119119 [ Pubmed: 35867819 DOI: doi:10.1073/pnas.2114119119 ]
subtomogram averaging of a HIV-1 immature Gag at 4.5 angstrom resolution
Ni T, Frosio T, Mendonca L, Sheng Y, Clare D, Himes BA, Zhang P
Nat Protoc (2022) 17 pp. 421-444 [ Pubmed: 35022621 DOI: doi:10.1038/s41596-021-00648-5 ]
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7bl5
Q-score: 0.363
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ DOI: doi:10.1016/j.molcel.2021.02.006 Pubmed: 33639093 ]
- 23s ribosomal rna (946 kDa, RNA from Escherichia coli str. K-12 substr. MG1655)
- Upf0307 protein yjga (21 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Ribosomal large subunit pseudouridine synthase d (37 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Assembly intermediate complex 3 (1 MDa, Complex from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Water (18 Da, Ligand)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Ribosomal silencing factor rsfs (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
Transcription termination complex 1
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Transcription termination complex (800 kDa, Complex from Escherichia coli)
Transcription termination intermediate complex 2
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Transcription termination complex (800 kDa, Complex from Escherichia coli)
Transcription termination intermediate complex 3
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Transcription termination complex (800 kDa, Complex from Escherichia coli)
Transcription termination interdmediate complex 4
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Transcription termination complex (800 kDa, Complex from Escherichia coli)
Transcription termination intermediate complex 5
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Transcription termination complex (800 kDa, Complex from Escherichia coli)
Transcription termination intermediate complex 3 delta NusG
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7adc
Q-score: 0.259
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Transcription termination intermediate complex 3 delta nusg (Complex from Escherichia coli)
- Beryllium trifluoride ion (66 Da, Ligand)
- Ntdna, tdna and rut rna (Complex from synthetic construct)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Ntdna (15 kDa, DNA from synthetic construct)
- Rut rna (31 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Transcription termination intermediate complex 3 delta nusg (800 kDa, Complex)
- Tdna (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)
Transcription termination intermediate complex IIIa
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7add
Q-score: 0.217
Said N, Hilal T, Sunday ND, Khatri A, Burger J, Mielke T, Belogurov GA, Loll B, Sen R, Artsimovitch I, Wahl MC
Science (2021) 371 [ DOI: doi:10.1126/science.abd1673 Pubmed: 33243850 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Ntdna, tdna and rut rna (Complex from synthetic construct)
- Transcription termination factor rho (47 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Ntdna (15 kDa, DNA from synthetic construct)
- Transcription termination intermediate complex iiia (800 kDa, Complex)
- Rut rna (31 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Transcription termination intermediate complex iiia (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Tdna (15 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Transcription termination/antitermination protein nusa (55 kDa, Protein from Escherichia coli)