CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8dwc
Q-score: 0.573
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8dwg
Q-score: 0.596
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 2-[(cyclopropanesulfonyl)amino]-n-(2-ethoxyphenyl)benzamide (360 Da, Ligand)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX1 with ligand Compound-16
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8dwh
Q-score: 0.51
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- N-{2-[(1-aminoisoquinolin-6-yl)oxy]-4-methylphenyl}-2-methoxybenzene-1-sulfonamide (435 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of full-length dimeric ClbP
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 7ul6
Q-score: 0.452
Velilla JA, Volpe MR, Kenney GE, Walsh Jr RM, Balskus EP, Gaudet R
Nat Chem Biol (2023) 19 pp. 151-158 [ Pubmed: 36253550 DOI: doi:10.1038/s41589-022-01142-z ]
- Clbp (110 kDa, Complex from Escherichia coli CFT073)
- Beta-lactamase (53 kDa, Protein from Escherichia coli CFT073)
The insulin receptor ectodomain in complex with a venom hybrid insulin analog - "head" region
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7mqo
Q-score: 0.44
Xiong X, Blakely A, Kim JH, Menting JG, Schafer IB, Schubert HL, Agrawal R, Gutmann T, Delaine C, Zhang YW, Artik GO, Merriman A, Eckert D, Lawrence MC, Coskun U, Fisher SJ, Forbes BE, Safavi-Hemami H, Hill CP, Chou DH
Nat Chem Biol (2022) 18 pp. 511-519 [ DOI: doi:10.1038/s41589-022-00981-0 Pubmed: 35289328 ]
- Insulin receptor ectodomain in complex with insulin analog vh-ins-hslq - focused refinement. (209 kDa, Complex from Homo sapiens)
- Insulin a chain (2 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Insulin b chain (2 kDa, Protein from Homo sapiens)
- Isoform short of insulin receptor (104 kDa, Protein from Homo sapiens)
The insulin receptor ectodomain in complex with four venom hybrid insulins - symmetric conformation
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7mqr
Q-score: 0.304
Xiong X, Blakely A, Kim JH, Menting JG, Schafer IB, Schubert HL, Agrawal R, Gutmann T, Delaine C, Zhang YW, Artik GO, Merriman A, Eckert D, Lawrence MC, Coskun U, Fisher SJ, Forbes BE, Safavi-Hemami H, Hill CP, Chou DH
Nat Chem Biol (2022) 18 pp. 511-519 [ DOI: doi:10.1038/s41589-022-00981-0 Pubmed: 35289328 ]
- Insulin receptor ectodomain in complex with insulin analog vh-ins-hslq - whole ectodomain. (209 kDa, Complex from Homo sapiens)
- Isoform short of insulin receptor (104 kDa, Protein from Homo sapiens)
- Insulin a chain (2 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Insulin b chain (2 kDa, Protein from Homo sapiens)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 7mqs
Q-score: 0.232
Xiong X, Blakely A, Kim JH, Menting JG, Schafer IB, Schubert HL, Agrawal R, Gutmann T, Delaine C, Zhang YW, Artik GO, Merriman A, Eckert D, Lawrence MC, Coskun U, Fisher SJ, Forbes BE, Safavi-Hemami H, Hill CP, Chou DH
Nat Chem Biol (2022) 18 pp. 511-519 [ DOI: doi:10.1038/s41589-022-00981-0 Pubmed: 35289328 ]
- Isoform short of insulin receptor (104 kDa, Protein from Homo sapiens)
- Insulin receptor ectodomain in complex with insulin analog vh-ins-hslq - asymmetric conformation. (209 kDa, Complex from Homo sapiens)
- Insulin b chain (2 kDa, Protein from Homo sapiens)
- Insulin a chain (2 kDa, Protein from Homo sapiens)
Substance P bound to active human neurokinin 1 receptor in complex with miniGs/q70
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7rmg
Q-score: 0.532
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ DOI: doi:10.1038/s41589-021-00890-8 Pubmed: 34711980 ]
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Substance p (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Substance p bound to neurokinin 1 receptor-minigs/q70 complex (Complex)
- Substance-p receptor (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Substance p (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s)/g(q) subunit alpha hybrid (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (26 kDa, Protein from Homo sapiens)
Substance P bound to active human neurokinin 1 receptor in complex with miniGs399
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7rmh
Q-score: 0.492
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ DOI: doi:10.1038/s41589-021-00890-8 Pubmed: 34711980 ]
- Substance p bound to neurokinin1 receptor-minigs399 complex (Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Substance p (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Substance-p receptor (Complex from Homo sapiens)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Minigs399 (Complex from Homo sapiens)
- Substance p (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
SP6-11 biased agonist bound to active human neurokinin 1 receptor in complex with miniGs/q70
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7rmi
Q-score: 0.458
Harris JA, Faust B, Gondin AB, Damgen MA, Suomivuori CM, Veldhuis NA, Cheng Y, Dror RO, Thal DM, Manglik A
Nat Chem Biol (2022) 18 pp. 109-115 [ DOI: doi:10.1038/s41589-021-00890-8 Pubmed: 34711980 ]
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Substance p 6-11 (739 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1/gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance-p receptor (47 kDa, Protein from Homo sapiens)
- Substance p bound to neurokinin 1 receptor-minigs/q70 complex (Complex)
- Substance-p receptor (Complex from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Substance p 6-11 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s)/g(q) subunit alpha hybrid (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (26 kDa, Protein from Homo sapiens)