Cryo-EM structure of ACh-bound M2R-Go signaling complex in S1 state
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7t8x
Q-score: 0.517
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of ACh-bound M2R-Go signaling complex in S2 state
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7t90
Q-score: 0.47
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of S1 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7t94
Q-score: 0.46
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2,muscarinic acetylcholine receptor m2 chimera (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Antibody fragment (27 kDa, Protein from Mus musculus)
- Water (18 Da, Ligand)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of S2 state ACh-bound M2R-Go signaling complex with a PAM
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7t96
Q-score: 0.466
Xu J, Wang Q, Hubner H, Hu Y, Niu X, Wang H, Maeda S, Inoue A, Tao Y, Gmeiner P, Du Y, Jin C, Kobilka BK
Nat Commun (2023) 14 pp. 376-376 [ DOI: doi:10.1038/s41467-022-35726-z Pubmed: 36690613 ]
- Muscarinic acetylcholine receptor m2 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Ach-bound m2r-goa-scfv16 complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Acetylcholine (146 Da, Ligand)
Cryo-EM structure of SARS-CoV spike receptor-binding domain in complex with sea lion ACE2
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7wsg
Q-score: 0.464
Li S, Yang R, Zhang D, Han P, Xu Z, Chen Q, Zhao R, Zhao X, Qu X, Zheng A, Wang L, Li L, Hu Y, Zhang R, Su C, Niu S, Zhang Y, Qi J, Liu K, Wang Q, Gao GF
Natl Sci Rev (2022) 9 pp. nwac122-nwac122 [ Pubmed: 36187898 DOI: doi:10.1093/nsr/nwac122 ]
- Zinc ion (65 Da, Ligand)
- Sea lion ace2 (Complex)
- Sars-cov spike receptor-binding domain in complex with sea lion ace2 (Complex from Mammalia)
- Sars-cov spike receptor-binding domain (Complex from Severe acute respiratory syndrome coronavirus)
- Angiotensin-converting enzyme (93 kDa, Protein from Mammalia)
- Spike protein s1 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus)
Cryo-EM structure of SARS-CoV-2 spike receptor-binding domain in complex with sea lion ACE2
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 7wsh
Q-score: 0.559
Li S, Yang R, Zhang D, Han P, Xu Z, Chen Q, Zhao R, Zhao X, Qu X, Zheng A, Wang L, Li L, Hu Y, Zhang R, Su C, Niu S, Zhang Y, Qi J, Liu K, Wang Q, Gao GF
Natl Sci Rev (2022) 9 pp. nwac122-nwac122 [ Pubmed: 36187898 DOI: doi:10.1093/nsr/nwac122 ]
- Zinc ion (65 Da, Ligand)
- Sea lion ace2 (Complex)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sars-cov-2 spike receptor-binding domain (Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike protein s1 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike receptor-binding domain in complex with sea lion ace2 (Complex from Mammalia)
- Angiotensin-converting enzyme (93 kDa, Protein from Mammalia)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7wse
Q-score: 0.504
Li S, Yang R, Zhang D, Han P, Xu Z, Chen Q, Zhao R, Zhao X, Qu X, Zheng A, Wang L, Li L, Hu Y, Zhang R, Su C, Niu S, Zhang Y, Qi J, Liu K, Wang Q, Gao GF
Natl Sci Rev (2022) 9 pp. nwac122-nwac122 [ Pubmed: 36187898 DOI: doi:10.1093/nsr/nwac122 ]
- Zinc ion (65 Da, Ligand)
- Angiotensin-converting enzyme (85 kDa, Protein from Mammalia)
- Sars-cov-2 spike receptor-binding domain complexed with its receptor minke whale ace2 (Complex from Mammalia)
- Spike protein s1 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Minke whale ace2 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 spike receptor-binding domain (Complex)
Cryo-EM structure of SARS-CoV spike receptor-binding domain in complex with minke whale ACE2
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7wsf
Q-score: 0.544
Li S, Yang R, Zhang D, Han P, Xu Z, Chen Q, Zhao R, Zhao X, Qu X, Zheng A, Wang L, Li L, Hu Y, Zhang R, Su C, Niu S, Zhang Y, Qi J, Liu K, Wang Q, Gao GF
Natl Sci Rev (2022) 9 pp. nwac122-nwac122 [ Pubmed: 36187898 DOI: doi:10.1093/nsr/nwac122 ]
- Zinc ion (65 Da, Ligand)
- Minke whale ace2 (Complex from Severe acute respiratory syndrome coronavirus)
- Angiotensin-converting enzyme (85 kDa, Protein from Mammalia)
- Sars-cov spike receptor-binding domain in complex with minke whale ace2 (Complex from Mammalia)
- Sars-cov spike receptor-binding domain (Complex)
- Spike protein s1 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus)
The complex structure of Omicron BA.1 RBD with BD604, S309,and S304
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 7x1m
Q-score: 0.552
Huang M, Wu L, Zheng A, Xie Y, He Q, Rong X, Han P, Du P, Han P, Zhang Z, Zhao R, Jia Y, Li L, Bai B, Hu Z, Hu S, Niu S, Hu Y, Liu H, Liu B, Cui K, Li W, Zhao X, Liu K, Qi J, Wang Q, Gao GF
Immunity (2022) 55 pp. 1501-1514.e3 [ DOI: doi:10.1016/j.immuni.2022.06.005 Pubmed: 35777362 ]
- S304 fab light chain (23 kDa, Protein from Homo sapiens)
- Spike protein s1 (23 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Bd604 fab, s309 fab and s304 fab complex with omicron ba.1 rbd (Complex)
- Omicron ba.1 rbd (Complex from Severe acute respiratory syndrome coronavirus 2)
- Bd-604 fab heavy chain (23 kDa, Protein from Homo sapiens)
- S309 fab heavy chain (24 kDa, Protein from Homo sapiens)
- Bd604 fab, s309 fab and s304 fab (Complex from Homo sapiens)
- S304 fab haavy chain (23 kDa, Protein from Homo sapiens)
- Bd-604 fab light chain (23 kDa, Protein from Homo sapiens)
- S309 fab light chain (23 kDa, Protein from Homo sapiens)
SARS-CoV-2 Omicron variant spike protein in complex with Fab XGv282
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7we7
Q-score: 0.315
Wang K, Jia Z, Bao L, Wang L, Cao L, Chi H, Hu Y, Li Q, Zhou Y, Jiang Y, Zhu Q, Deng Y, Liu P, Wang N, Wang L, Liu M, Li Y, Zhu B, Fan K, Fu W, Yang P, Pei X, Cui Z, Qin L, Ge P, Wu J, Liu S, Chen Y, Huang W, Wang Q, Qin CF, Wang Y, Qin C, Wang X
Nature (2022) 603 pp. 919-925 [ Pubmed: 35090164 DOI: doi:10.1038/s41586-022-04466-x ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- The light chain of fab 282 (11 kDa, Protein from Homo sapiens)
- Heavy chain of fab 282 (13 kDa, Protein from Homo sapiens)
- Fab 282 (Complex from Homo sapiens)
- Spike glycoprotein (141 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 omicron mutation spike protein (Complex)
- Sars-cov-2 omicron mutation spike protein in complex with fab 265 (Complex from Homo sapiens)