Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 7e1w
Q-score: 0.569
Zhou S, Wang W, Zhou X, Zhang Y, Lai Y, Tang Y, Xu J, Li D, Lin J, Yang X, Ran T, Chen H, Guddat LW, Wang Q, Gao Y, Rao Z, Gong H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.69418 Pubmed: 34819223 ]
- Cardiolipin (1 kDa, Ligand)
- Menaquinone-9 (785 Da, Ligand)
- Cytochrome bc1 complex cytochrome b subunit (61 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- 6-chloranyl-2-ethyl-n-[[4-[4-[4-(trifluoromethyloxy)phenyl]piperidin-1-yl]phenyl]methyl]imidazo[1,2-a]pyridine-3-carboxamide (557 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- (2r)-2-(hexadecanoyloxy)-3-{[(s)-hydroxy{[(1r,2r,3r,4r,5r,6s)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9s)-9-methyloctadecanoate (853 Da, Ligand)
- Cytochrome c oxidase subunit 3 (22 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Cytochrome bc1 complex cytochrome c subunit (29 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
- Cytochrome c oxidase subunit 1 (64 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Uncharacterized protein msmeg_4692/msmei_4575 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Hybrid respiratory supercomplex consisting of mycobacterium tuberculosis complexiii and mycobacterium smegmatis complexiv in the presence of q203 (800 kDa, Complex from Mycolicibacterium smegmatis MC2 51)
- Palmitic acid (256 Da, Ligand)
- Cytochrome c oxidase polypeptide 4 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Cytochrome c oxidase subunit ctaj (8 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Heme-a (852 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Prokaryotic respiratory supercomplex associate factor 1 prsaf1 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Heme c (618 Da, Ligand)
- Water (18 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome bc1 complex rieske iron-sulfur subunit (46 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Cytochrome c oxidase subunit 2 (38 kDa, Protein from Mycobacterium smegmatis MC2 51)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 7e1x
Q-score: 0.513
Zhou S, Wang W, Zhou X, Zhang Y, Lai Y, Tang Y, Xu J, Li D, Lin J, Yang X, Ran T, Chen H, Guddat LW, Wang Q, Gao Y, Rao Z, Gong H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.69418 Pubmed: 34819223 ]
- Mycobacterium smegmatis (Complex from Mycolicibacterium smegmatis MC2 155)
- Cardiolipin (1 kDa, Ligand)
- Menaquinone-9 (785 Da, Ligand)
- Cytochrome bc1 complex cytochrome b subunit (61 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- (2r)-2-(hexadecanoyloxy)-3-{[(s)-hydroxy{[(1r,2r,3r,4r,5r,6s)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9s)-9-methyloctadecanoate (853 Da, Ligand)
- Cytochrome c oxidase subunit 3 (22 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Cytochrome bc1 complex cytochrome c subunit (29 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
- Cytochrome c oxidase subunit 1 (64 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Uncharacterized protein msmeg_4692/msmei_4575 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Palmitic acid (256 Da, Ligand)
- Cytochrome c oxidase polypeptide 4 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Hybrid respiratory supercomplex consisting of mycobacterium tuberculosis complexiii and mycobacterium smegmatis complexiv in presence of tb47 (800 kDa, Complex from Mycolicibacterium smegmatis MC2 51)
- Cytochrome c oxidase subunit ctaj (8 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Heme-a (852 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Prokaryotic respiratory supercomplex associate factor 1 prsaf1 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Heme c (618 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome bc1 complex rieske iron-sulfur subunit (46 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- 5-methoxy-2-methyl-~{n}-[[4-[4-[4-(trifluoromethyloxy)phenyl]piperidin-1-yl]phenyl]methyl]pyrazolo[1,5-a]pyridine-3-carboxamide (538 Da, Ligand)
- Cytochrome c oxidase subunit 2 (38 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Mycobacterium tuberculosis complexiii (Complex)
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7e1v
Q-score: 0.614
Zhou S, Wang W, Zhou X, Zhang Y, Lai Y, Tang Y, Xu J, Li D, Lin J, Yang X, Ran T, Chen H, Guddat LW, Wang Q, Gao Y, Rao Z, Gong H
eLife (2021) 10 [ DOI: doi:10.7554/eLife.69418 Pubmed: 34819223 ]
- Cardiolipin (1 kDa, Ligand)
- Menaquinone-9 (785 Da, Ligand)
- Cytochrome bc1 complex cytochrome b subunit (61 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- (2r)-2-(hexadecanoyloxy)-3-{[(s)-hydroxy{[(1r,2r,3r,4r,5r,6s)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl (9s)-9-methyloctadecanoate (853 Da, Ligand)
- Cytochrome c oxidase subunit 3 (22 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Mycobacterium tuberculosis complexiii (Complex from Mycobacterium tuberculosis H37Rv)
- Cytochrome bc1 complex cytochrome c subunit (29 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- (2r)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (e)-octadec-9-enoate (717 Da, Ligand)
- Cytochrome c oxidase subunit 1 (64 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Uncharacterized protein msmeg_4692/msmei_4575 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Palmitic acid (256 Da, Ligand)
- Cytochrome c oxidase polypeptide 4 (15 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Cytochrome c oxidase subunit ctaj (8 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Heme-a (852 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Prokaryotic respiratory supercomplex associate factor 1 prsaf1 (11 kDa, Protein from Mycolicibacterium smegmatis MC2 51)
- Heme c (618 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome bc1 complex rieske iron-sulfur subunit (46 kDa, Protein from Mycobacterium tuberculosis H37Rv)
- Cytochrome c oxidase subunit 2 (38 kDa, Protein from Mycobacterium smegmatis MC2 51)
- Mycobacterium smegmatis complexiv (Complex)
- Apo hybrid respiratory supercomplex consisting of mycobacterium tuberculosis complexiii and mycobacterium smegmatis complexiv (800 kDa, Complex from Mycolicibacterium smegmatis MC2 51)
Structure of Mycobacterium smegmatis bd complex in the apo-form.
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7d5i
Q-score: 0.597
Wang W, Gao Y, Tang Y, Zhou X, Lai Y, Zhou S, Zhang Y, Yang X, Liu F, Guddat LW, Wang Q, Rao Z, Gong H
Nat Commun (2021) 12 pp. 4621-4621 [ DOI: doi:10.1038/s41467-021-24924-w Pubmed: 34330928 ]
- Integral membrane cytochrome d ubiquinol oxidase (subunit ii) cydb (39 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Cytochrome d ubiquinol oxidase subunit 1 (53 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Heme b/c (618 Da, Ligand)
- Cis-heme d hydroxychlorin gamma-spirolactone (632 Da, Ligand)
- Cryo-em structure of mycobacterium smegmatis bd complex (Complex from Mycolicibacterium smegmatis MC2 155)
Cryo-EM structure of the encapsulin shell from Mycobacterium smegmatis
Cryo-EM structure of the encapsulated DyP-type peroxidase from Mycobacterium smegmatis
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7bwr
Q-score: 0.419
Zhang L, Zhao Y, Gao R, Li J, Yang X, Gao Y, Zhao W, Gurcha SS, Veerapen N, Batt SM, Besra KK, Xu W, Bi L, Zhang X, Guddat LW, Yang H, Wang Q, Besra GS, Rao Z
Protein Cell (2020) 11 pp. 505-517 [ DOI: doi:10.1007/s13238-020-00726-6 Pubmed: 32363534 ]
- [(2z,6e,10e,14z,18e,22z,26z)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl] [(2s,3s,4s,5r)-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl] hydrogen phosphate (911 Da, Ligand)
- Meromycolate extension acyl carrier protein (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterial arabinosyltransferase complex embb2-acpm2 (254 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
- Integral membrane indolylacetylinositol arabinosyltransferase embb (116 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Calcium ion (40 Da, Ligand)
Mycobacterium smegmatis arabinosyltransferase complex EmbB2-AcpM2 in symmetric "resting state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7bx8
Q-score: 0.443
Zhang L, Zhao Y, Gao R, Li J, Yang X, Gao Y, Zhao W, Gurcha SS, Veerapen N, Batt SM, Besra KK, Xu W, Bi L, Zhang X, Guddat LW, Yang H, Wang Q, Besra GS, Rao Z
Protein Cell (2020) 11 pp. 505-517 [ DOI: doi:10.1007/s13238-020-00726-6 Pubmed: 32363534 ]
- Mycobacterial arabinosyltransferase complex embb2-acpm2 (254 MDa, Complex from Mycolicibacterium smegmatis MC2 155)
- Integral membrane indolylacetylinositol arabinosyltransferase embb (116 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Meromycolate extension acyl carrier protein (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7bvc
Q-score: 0.564
Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z
Science (2020) 368 pp. 1211-1219 [ DOI: doi:10.1126/science.aba9102 Pubmed: 32327601 ]
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Meromycolate extension acyl carrier protein (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Acpm (Complex from Mycolicibacterium smegmatis MC2 155)
- Mycobacterium smegmatis arabinosyltransferase emba-embb-acpm2 in complex with ethambutol (Complex)
- Integral membrane indolylacetylinositol arabinosyltransferase embb (119 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- [(2z,6e,10e,14z,18e,22z,26z)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl] [(2s,3s,4s,5r)-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl] hydrogen phosphate (911 Da, Ligand)
- Emba-embb (Complex from Mycolicibacterium smegmatis MC2 155)
- Calcium ion (40 Da, Ligand)
- Integral membrane indolylacetylinositol arabinosyltransferase emba (116 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Ethambutol (204 Da, Ligand)
- 4'-phosphopantetheine (358 Da, Ligand)
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7bve
Q-score: 0.552
Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z
Science (2020) 368 pp. 1211-1219 [ DOI: doi:10.1126/science.aba9102 Pubmed: 32327601 ]
- Phosphate ion (94 Da, Ligand)
- Mycobacterium smegmatis arabinosyltransferase embc2-acpm2 in complex with ethambutol (Complex)
- Meromycolate extension acyl carrier protein (10 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Embc (Complex from Mycolicibacterium smegmatis MC2 155)
- Acpm (Complex from Mycolicibacterium smegmatis MC2 155)
- N~3~-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-n-(2-sulfanylethyl)-beta-alaninamide (358 Da, Ligand)
- Integral membrane indolylacetylinositol arabinosyltransferase embc (116 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Calcium ion (40 Da, Ligand)
- Ethambutol (204 Da, Ligand)