ATP-bound IstB in complex to duplex DNA
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8q3w
Q-score: 0.365
de la Gandara A, Spinola-Amilibia M, Araujo-Bazan L, Nunez-Ramirez R, Berger JM, Arias-Palomo E
Nature (2024) 630 pp. 1003-1011 [ Pubmed: 38926614 DOI: doi:10.1038/s41586-024-07550-6 ]
- Dna (48-mer) traget dna rv (18 kDa, DNA from Geobacillus stearothermophilus)
- Insertion sequence is5376 putative atp-binding protein (Complex from Geobacillus stearothermophilus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (48-mer) target dna fw (18 kDa, DNA from Geobacillus stearothermophilus)
- Dna (48-mer) target dna fw and rv (Complex from Geobacillus stearothermophilus)
- Istb transposition regulator aaa+ atpase bound to target dna (337 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Insertion sequence is5376 putative atp-binding protein (29 kDa, Protein from Geobacillus stearothermophilus)
IstA-IstB(E167Q) Strand Transfer Complex
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 8q4d
Q-score: 0.216
de la Gandara A, Spinola-Amilibia M, Araujo-Bazan L, Nunez-Ramirez R, Berger JM, Arias-Palomo E
Nature (2024) 630 pp. 1003-1011 [ Pubmed: 38926614 DOI: doi:10.1038/s41586-024-07550-6 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Putative transposase for insertion sequence element is5376 (44 kDa, Protein from Geobacillus stearothermophilus)
- Dna (Complex from Geobacillus stearothermophilus)
- Dna (58-mer) / target-reverse complement (17 kDa, DNA from Geobacillus stearothermophilus)
- Ista-istb(e167q) strand transfer complex (952 kDa, Complex)
- Ista-istb(e167q) strand (Complex from Geobacillus stearothermophilus)
- Insertion sequence is5376 putative atp-binding protein (28 kDa, Protein from Geobacillus stearothermophilus)
- Dna (58-mer) / tir non-transferred strand (17 kDa, DNA from Geobacillus stearothermophilus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (118-mer) / tir-transferred strand (36 kDa, DNA from Geobacillus stearothermophilus)
IstA transposase cleaved donor complex
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 8b4h
Q-score: 0.364
Spinola-Amilibia M, Araujo-Bazan L, de la Gandara A, Berger JM, Arias-Palomo E
Nat Commun (2023) 14 pp. 2335-2335 [ Pubmed: 37087515 DOI: doi:10.1038/s41467-023-38071-x ]
- Putative transposase for insertion sequence element is5376 (47 kDa, Protein from Geobacillus stearothermophilus)
- Dna (55-mer) / right is21 transposon end (insertion sequence is5376) (17 kDa, DNA from Geobacillus stearothermophilus)
- Ista transposase cleaved donor complex (225 kDa, Complex from Geobacillus stearothermophilus)
- Magnesium ion (24 Da, Ligand)
- Dna (57-mer) / right is21 transposon end (insertion sequence is5376) (18 kDa, DNA from Geobacillus stearothermophilus)