Cryo-EM structure of cyanobacterial photosystem I in the presence of ferredoxin and cytochrome c6
Resolution: 1.97 Å
EM Method: Single-particle
Fitted PDBs: 7fix
Q-score: 0.651
Li J, Hamaoka N, Makino F, Kawamoto A, Lin Y, Rogner M, Nowaczyk MM, Lee YH, Namba K, Gerle C, Kurisu G
Commun Biol (2022) 5 pp. 951-951 [ Pubmed: 36097054 DOI: doi:10.1038/s42003-022-03926-4 ]
- Phylloquinone (450 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (83 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Ferredoxin-1 (10 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Beta-carotene (536 Da, Ligand)
- Photosystem i reaction center subunit viii (4 kDa, Protein from Thermosynechococcus vestitus BP-1)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Cryo-em map of cyanobacterial photosystem i in the presence of ferredoxin and cytochrome c6 (1 MDa, Complex from Thermosynechococcus elongatus BP-1)
- Photosystem i reaction center subunit iv (8 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Water (18 Da, Ligand)
- Ga-substituted ferredoxin (Complex from Thermosynechococcus elongatus BP-1)
- Photosystem i trimer (Complex)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Chlorophyll a isomer (893 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Photosystem i reaction center subunit ix (4 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Calcium ion (40 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Photosystem i reaction center subunit iii (19 kDa, Protein from Thermosynechococcus vestitus BP-1)
- Photosystem i reaction center subunit psak (8 kDa, Protein from Thermosynechococcus vestitus BP-1)
- [2ga-2s] cluster (203 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Unknown ligand (40 Da, Ligand)
- Photosystem i 4.8k protein (4 kDa, Protein from Thermosynechococcus vestitus BP-1)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 7nho
Q-score: 0.501
Zabret J, Bohn S, Schuller SK, Arnolds O, Moller M, Meier-Credo J, Liauw P, Chan A, Tajkhorshid E, Langer JD, Stoll R, Krieger-Liszkay A, Engel BD, Rudack T, Schuller JM, Nowaczyk MM
Nat Plants (2021) 7 pp. 524-538 [ DOI: doi:10.1038/s41477-021-00895-0 Pubmed: 33846594 ]
- Photosystem ii reaction center protein m (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Photosystem ii reaction center protein i (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem ii reaction center protein h (7 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp43 reaction center protein (50 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Cytochrome b559 subunit alpha (9 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Bicarbonate ion (61 Da, Ligand)
- Psii-m (Complex from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp47 reaction center protein (56 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein ycf12 (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii protein d1 1 (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Fe (iii) ion (55 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Photosystem ii reaction center x protein (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Cytochrome b559 subunit beta (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein z (6 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein t (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Pheophytin a (871 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem ii reaction center protein l (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii d2 protein (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Photosystem ii reaction center protein k (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
Structure of PSII-I (PSII with Psb27, Psb28, and Psb34)
Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7nhp
Q-score: 0.469
Zabret J, Bohn S, Schuller SK, Arnolds O, Moller M, Meier-Credo J, Liauw P, Chan A, Tajkhorshid E, Langer JD, Stoll R, Krieger-Liszkay A, Engel BD, Rudack T, Schuller JM, Nowaczyk MM
Nat Plants (2021) 7 pp. 524-538 [ DOI: doi:10.1038/s41477-021-00895-0 Pubmed: 33846594 ]
- Photosystem ii reaction center protein m (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Photosystem ii reaction center protein i (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem ii lipoprotein psb27 (15 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein h (7 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Psii-i (Complex from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp43 reaction center protein (50 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Cytochrome b559 subunit alpha (9 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp47 reaction center protein (56 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein ycf12 (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii protein d1 1 (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem ii reaction center psb28 protein (13 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Cytochrome b559 subunit beta (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Chloride ion (35 Da, Ligand)
- Photosystem ii reaction center x protein (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Fe (iii) ion (55 Da, Ligand)
- Photosystem ii reaction center protein z (6 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein t (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Pheophytin a (871 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem ii reaction center protein l (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Tsl0063 protein (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii d2 protein (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Photosystem ii reaction center protein k (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
Structure of PSII-I prime (PSII with Psb28, and Psb34)
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 7nhq
Q-score: 0.465
Zabret J, Bohn S, Schuller SK, Arnolds O, Moller M, Meier-Credo J, Liauw P, Chan A, Tajkhorshid E, Langer JD, Stoll R, Krieger-Liszkay A, Engel BD, Rudack T, Schuller JM, Nowaczyk MM
Nat Plants (2021) 7 pp. 524-538 [ DOI: doi:10.1038/s41477-021-00895-0 Pubmed: 33846594 ]
- Photosystem ii reaction center protein m (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Photosystem ii reaction center protein i (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 2,3-dimethyl-5-(3,7,11,15,19,23,27,31,35-nonamethyl-2,6,10,14,18,22,26,30,34-hexatriacontanonaenyl-2,5-cyclohexadiene-1,4-dione-2,3-dimethyl-5-solanesyl-1,4-benzoquinone (749 Da, Ligand)
- Beta-carotene (536 Da, Ligand)
- Photosystem ii reaction center protein h (7 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp43 reaction center protein (50 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Cytochrome b559 subunit alpha (9 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii cp47 reaction center protein (56 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein ycf12 (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii protein d1 1 (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem ii reaction center psb28 protein (13 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Fe (iii) ion (55 Da, Ligand)
- Cytochrome b559 subunit beta (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center x protein (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Chloride ion (35 Da, Ligand)
- Photosystem ii reaction center protein z (6 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Psii-i prime (Complex from Thermosynechococcus elongatus BP-1)
- Photosystem ii reaction center protein t (3 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Pheophytin a (871 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
- Photosystem ii reaction center protein l (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Tsl0063 protein (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem ii d2 protein (39 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Photosystem ii reaction center protein k (5 kDa, Protein from Thermosynechococcus elongatus BP-1)
Cyanobacterial PSI Monomer from T. elongatus by Single Particle CRYO-EM at 3.2 A Resolution
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6lu1
Q-score: 0.582
Coruh O, Frank A, Tanaka H, Kawamoto A, El-Mohsnawy E, Kato T, Namba K, Gerle C, Nowaczyk MM, Kurisu G
Commun Biol (2021) 4 pp. 304-304 [ DOI: doi:10.1038/s42003-021-01808-9 Pubmed: 33686186 ]
- Photosystem i p700 chlorophyll a apoprotein a1 (83 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Water (18 Da, Ligand)
- Photosystem i reaction center subunit xi (16 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Photosystem i reaction center subunit iv (8 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Photosystem i reaction center subunit ix (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Phylloquinone (450 Da, Ligand)
- Photosystem i iron-sulfur center (8 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Photosystem i reaction center subunit xii (3 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Cyanobacterial psi monomer from t. elongatus (350 MDa, Complex from Thermosynechococcus elongatus BP-1)
- Photosystem i reaction center subunit iii (17 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Photosystem i reaction center subunit viii (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Chlorophyll a (893 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosystem i reaction center subunit ii (15 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Beta-carotene (536 Da, Ligand)
- Photosystem i p700 chlorophyll a apoprotein a2 (83 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
Structure of the NDH-1MS complex from Thermosynechococcus elongatus
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6tjv
Q-score: 0.529
Schuller JM, Saura P, Thiemann J, Schuller SK, Gamiz-Hernandez AP, Kurisu G, Nowaczyk MM, Kaila VRI
Nat Commun (2020) 11 pp. 494-494 [ Pubmed: 31980611 DOI: doi:10.1038/s41467-020-14347-4 ]
- Nad(p)h-quinone oxidoreductase subunit l (8 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Iron/sulfur cluster (351 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nad(p)h-quinone oxidoreductase subunit i (22 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit j (19 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit k (25 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Beta-carotene (536 Da, Ligand)
- Tlr0906 protein (51 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nadh dehydrogenase subunit 6 (21 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit 1 (40 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit 4l (11 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nadh dehydrogenase subunit 4 (53 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit h (45 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Water (18 Da, Ligand)
- Nad(p)h-quinone oxidoreductase subunit n (16 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- 1,2-di-o-acyl-3-o-[6-deoxy-6-sulfo-alpha-d-glucopyranosyl]-sn-glycerol (795 Da, Ligand)
- Nad(p)h-quinone oxidoreductase subunit o (7 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nadh dehydrogenase subunit 5 (66 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit 2 (55 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Nad(p)h-quinone oxidoreductase subunit 3 (15 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Zinc ion (65 Da, Ligand)
- Ndh-1ms (Complex from Thermosynechococcus elongatus BP-1)
- Tlr0636 protein (12 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Chlorophyll a (893 Da, Ligand)
- Nad(p)h-quinone oxidoreductase subunit m (12 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
- Digalactosyl diacyl glycerol (dgdg) (949 Da, Ligand)
- Tll0220 protein (15 kDa, Protein from Thermosynechococcus elongatus (strain BP-1))
Structure of the photosynthetic complex I from Thermosynechococcus elongatus
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 6hum
Q-score: 0.482
Schuller JM, Birrell JA, Tanaka H, Konuma T, Wulfhorst H, Cox N, Schuller SK, Thiemann J, Lubitz W, Setif P, Ikegami T, Engel BD, Kurisu G, Nowaczyk MM
Science (2019) 363 pp. 257-260 [ DOI: doi:10.1126/science.aau3613 Pubmed: 30573545 ]
- Nad(p)h-quinone oxidoreductase subunit 4l (11 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit h (45 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit l (8 kDa, Protein from Thermosynechococcus elongatus BP-1)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- Nad(p)h-quinone oxidoreductase chain 4 1 (57 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit 2 (55 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit 3 (15 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Beta-carotene (536 Da, Ligand)
- Nadh dehydrogenase subunit 5 (72 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Proton-translocating nadh-quinone dehydrogenase subunit p ndhp (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit k (25 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Proton-translocating nadh-quinone dehydrogenase subunit q ndhq (4 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit n (16 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit 1 (40 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit o (7 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nadh dehydrogenase subunit 6 (21 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit m (12 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit i (22 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Tlr0636 protein (12 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Nad(p)h-quinone oxidoreductase subunit j (19 kDa, Protein from Thermosynechococcus elongatus BP-1)
- Iron/sulfur cluster (351 Da, Ligand)
- Photosynthetic complex i (Complex from Thermosynechococcus elongatus (strain BP-1))