Structural basis for DNA strand separation by a hexameric replicative helicase
Chaban Y, Stead JA, Ryzhenkova K, Whelan F, Lamber K, Antson A, Sanders CM, Orlova EV
Nucleic Acids Res (2015) 43 pp. 8551-8563 [ DOI: doi:10.1093/nar/gkv778 Pubmed: 26240379 ]
- Monovalent tetrameric streptavidin (53 kDa, Protein from Streptomyces avidinii)
- Full-length hexameric e1 helicase (410 kDa, Protein from Bovine papillomavirus)
- Full-length e1 helicase-dna complex labelled with monovalent tetrameric streptavidin (mts) on the dsdna end of the dna fork (460 kDa, Sample)
Structural basis for DNA strand separation by a hexameric replicative helicase
Chaban Y, Stead JA, Ryzhenkova K, Whelan F, Lamber K, Antson A, Sanders CM, Orlova EV
Nucleic Acids Res (2015) 43 pp. 8551-8563 [ DOI: doi:10.1093/nar/gkv778 Pubmed: 26240379 ]
- Igg2a-kappa 26-10 fab (30 kDa, Protein from Mus musculus)
- Monovalent tetrameric streptavidin (50 kDa, Protein from Streptomyces avidinii)
- Full-length e1 helicase-dna complex labelled with monovalent tetrameric streptavidin (mts) on the dsdna end and with fab on the 5' ssdna end (490 kDa, Sample)
- Full-length hexametric e1 helicase complex (410 kDa, Protein from Bovine papillomavirus)
Full-length structure of the bacterial pKM101 type IV secretion system core complex
Resolution: 12.4 Å
EM Method: Single-particle
Fitted PDBs: 2ypw
Q-score: -0.007
Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV
EMBO J (2013) 32 pp. 1195-1204 [ DOI: doi:10.1038/emboj.2013.58 Pubmed: 23511972 ]
- Trao (30 kDa, Protein from Escherichia coli)
- Tran/trao/traf complex encoded by pkm101 (1 MDa, Sample)
- Traf (40 kDa, Protein from Escherichia coli)
- Tran (5 kDa, Protein from Escherichia coli)
Resolution: 8.5 Å
EM Method: Single-particle
Fitted PDBs: 3zbi, 3zbj
Q-score: 0.0195
Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV
EMBO J (2013) 32 pp. 1195-1204 [ DOI: doi:10.1038/emboj.2013.58 Pubmed: 23511972 ]
- Tran/trao/traf complex encoded by pkm101 digested with 0.02 mg ml-1 of elastase for 40 min at room temperature (880 kDa, Sample)
- Trao (30 kDa, Protein from Escherichia coli)
- Traf (40 kDa, Protein from Escherichia coli)
- Tran (5 kDa, Protein from Escherichia coli)
Resolution: 17.0 Å
EM Method: Single-particle
Fitted PDBs: 2j9i
Q-score: 0.033
Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G
Structure (2006) 14 pp. 1823-1834 [ Pubmed: 17161372 DOI: doi:10.1016/j.str.2006.10.008 ]
- Lengsin (510 kDa, Sample)
- Lengsin (Protein from Mus musculus)
Structure of the Escherichia coli ribosomal termination complex with release factor 2.
Resolution: 14.0 Å
EM Method: Single-particle
Fitted PDBs: 1ml5
Q-score: 0.013
Klaholz BP, Pape T, Zavialov AV, Myasnikov AG, Orlova EV, Vestergaard B, Ehrenberg M, van Heel M
Nature (2003) 421 pp. 90-94 [ DOI: doi:10.1038/nature01225 Pubmed: 12511961 ]
- Release factor 2 (40 kDa, Protein from Escherichia coli BL21(DE3))
- Ribosome (2 MDa, Complex from Escherichia coli)
- Release factor rf2 bound to e. coli ribosomes (2 MDa, Sample)